Bhadra S

References (5)

Title : Anti-cholinesterase activity of the standardized extract of Syzygium aromaticum L - Dalai_2014_Pharmacogn.Mag_10_S276
Author(s) : Dalai MK , Bhadra S , Chaudhary SK , Bandyopadhyay A , Mukherjee PK
Ref : Pharmacogn Mag , 10 :S276 , 2014
Abstract : BACKGROUND: Clove (Syzygium aromaticum) is a well-known culinary spice with strong aroma; contains a high amount of oil known as clove oil. The major phyto-constituent of the clove oil is eugenol. Clove and its oil possess various medicinal uses in indigenous medicine as an antiseptic, anti-oxidant, analgesic and neuroprotective properties. Thus, it draws much attention among researchers from pharmaceutical, food and cosmetic industries. OBJECTIVE: The aim of the present study was to determine the anti-cholinesterase activity of the methanol extract of clove, its oil and eugenol. MATERIALS AND
METHODS: In vitro anti-cholinesterase activity of S. aromaticum was performed by a thin layer chromatography bio autography, 96 well micro titer plate and kinetic methods. Reverse phase high performance liquid chromatography (RP-HPLC) analysis was carried out to identify the biomarker compound eugenol in clove oil.
RESULTS: Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition study revealed that eugenol possess better inhibition of the enzymes than extract and oil. Clove extract, its oil and eugenol showed better inhibition of AChE than BChE. Polyphenolic compound eugenol was detected through RP-HPLC analysis. The content of eugenol in essential oil was found to be 0.5 mug/ml. Kinetic analysis of the cholinesterase inhibition study of the extract; clove oil and eugenol have shown that they possess mixed type of inhibition for AChE and non-competitive type of inhibition for BChE. CONCLUSION: These results might be useful in explaining the effect of clove as anti-cholinesterase agent for the management of cognitive ailments like Alzheimer's disease.
ESTHER : Dalai_2014_Pharmacogn.Mag_10_S276
PubMedSearch : Dalai_2014_Pharmacogn.Mag_10_S276
PubMedID: 24991103

Title : Cholinesterase inhibition activity of Marsilea quadrifolia Linn. an edible leafy vegetable from West Bengal, India - Bhadra_2012_Nat.Prod.Res_26_1519
Author(s) : Bhadra S , Mukherjee PK , Bandyopadhyay A
Ref : Nat Prod Res , 26 :1519 , 2012
Abstract : Maesilea quadrifolia Linn. (Marsileaceae) is a leafy vegetable well known in India. The current study aims to explore the phytochemical profile of M. quadrifolia and investigate its anti-cholinesterase potential. The methanol extract of the plant was subjected to qualitative and quantitative phytochemical screening (total alkaloidal content, saponin content and phenol content) and its anti-cholinesterase potential was tested by TLC bioautography and other screening methods using acytylcholinesterase (AChE) and butyrylcholinesterase (BChE). The study revealed that the extract contains various classes of phytoconstituents including steroids, saponins, alkaloids and other polyphenols. Total alkaloid, phenolic and saponin contents were found to be 19.3 mg g(-)(1) and 158.5 +/- 1.02 mg g(-)(1) as gallic acid equivalents and 2.63 mg g(-)(1) of the extract, respectively. The TLC bioautography method exhibited the inhibition of both enzymes. In a microtiter plate assay, the IC(5)(0) values of the extract for AChE and BChE were found to be 51.89 +/- 0.24 microg mL(-)(1) and 109.43 +/- 2.82 microg mL(-)(1), respectively. These findings suggest that M. quadrifolia is a potential lead as an AChE and BChE inhibitor, which may be useful in the management of Alzheimer's disease.
ESTHER : Bhadra_2012_Nat.Prod.Res_26_1519
PubMedSearch : Bhadra_2012_Nat.Prod.Res_26_1519
PubMedID: 21978132

Title : Anticholinesterase activity of standardized extract of Illicium verum Hook. f. fruits - Bhadra_2011_Fitoterapia_82_342
Author(s) : Bhadra S , Mukherjee PK , Kumar NS , Bandyopadhyay A
Ref : Fitoterapia , 82 :342 , 2011
Abstract : Illicium verum is a well known spice in traditional Indian system for its therapeutic potential. The present study was aimed to evaluate the acetylcholinesterase (AChE) and butyrylcholinesterase inhibitory (BChE) activity of standardized extracts of I. verum and its oil. Present study confirmed that anethole contributed to the anticholinesterase activity of I. verum, with more specificity towards AChE. IC(50) for AChE and BChE inhibitory activity of anethole was 39.89+/-0.32 mug/mL and 75.35+/-1.47 mug/mL, whereas for the oil, 36.00+/-0.44 mug/mL and 70.65+/-0.96 mug/mL respectively. Therefore I. verum can be a good lead as anti-cholinesterase agent from natural resources.
ESTHER : Bhadra_2011_Fitoterapia_82_342
PubMedSearch : Bhadra_2011_Fitoterapia_82_342
PubMedID: 21075180

Title : Acetylcholinesterase enzyme inhibitory potential of standardized extract of Trigonella foenum graecum L and its constituents - Satheeshkumar_2010_Phytomedicine_17_292
Author(s) : Satheeshkumar N , Mukherjee PK , Bhadra S , Saha BP
Ref : Phytomedicine , 17 :292 , 2010
Abstract : Ethno pharmacological approach has provided several leads to identify potential new drugs from plant sources, including those for memory disorders. Acetylcholinesterase inhibitors (AChEI) give a symptomatic relief to some of the clinical manifestations of the disease. The main objective of this study is to standardize the extract of Trigonella foenum graecum L with trigonelline by HPTLC method and determine the in vitro AChE inhibitory activity of Trigonella foenum graecum L and its constituents using galanthamine as a reference. Different concentrations of hydro alcoholic extract of Trigonella foenum graecum and trigonelline were subjected to HPTLC analysis using the mobile phase n propanol, methanol and water (4:1:2, v/v). The R(f) of trigonelline was found to be 0.43, and the correlation coefficient of 0.99 was indicative of good linear dependence of peak area on concentration. The concentration of trigonelline was found to be 13mgg(-1)w/w in the hydro alcoholic extract of Trigonella foenum graecum. The AChE inhibitory activity of crude fenugreek seed extracts, fractions and trigonelline was evaluated using Ellman's method in 96-well micro plate's assay and TLC bioassay detection. The ethyl acetate fraction of the alcohol extract (IC50 53.00 +/- 17.33microg/ml), and total alkaloid fraction (IC50 9.23+/-6.08microg/ml) showed potential AChE inhibition. Trigonelline showed IC50 233+/-0.12microM. Galanthamine was used as standard and it showed inhibition of acetyl cholinesterase with an IC50 value of 1.27+/-0.21microM.
ESTHER : Satheeshkumar_2010_Phytomedicine_17_292
PubMedSearch : Satheeshkumar_2010_Phytomedicine_17_292
PubMedID: 19576740

Title : Acetylcholinesterase inhibitory potential of a carbazole alkaloid, mahanimbine, from Murraya koenigii - Kumar_2010_Phytother.Res_24_629
Author(s) : Kumar NS , Mukherjee PK , Bhadra S , Saha BP , Pal BC
Ref : Phytother Res , 24 :629 , 2010
Abstract : In the search for acetylcholinesterase (AChE) inhibitors from Indian medicinal plants, via bioassay-guided isolation, a carbazole alkaloid, mahanimbine [3, 5-dimethyl-3-(4- methylpent-3-enyl)-11H-pyrano [5, 6-a] carbazole], was isolated from the petroleum ether extract of the leaves of Murraya koenigii. Inhibition of AChE was evaluated based on Ellman's method using 96-well microplate readers. Mahanimbine inhibited AChE activity in a dose-dependent manner with an IC(50) value of 0.03 +/- 0.09 mg/mL, while galantamine was used as a standard. The AChE inhibitory activity of this carbazole alkaloid has not been reported so far, and this study is the first to reveal this activity in carbazole alkaloid mahanimbine, isolated from Murraya koenigii.
ESTHER : Kumar_2010_Phytother.Res_24_629
PubMedSearch : Kumar_2010_Phytother.Res_24_629
PubMedID: 19943242