Havel J

References (4)

Title : Determination of physostigmine and pyridostigmine in pharmaceutical formulations by capillary electrophoresis - Havel_2002_J.Capill.Electrophor.Microchip.Technol_7_107
Author(s) : Havel J , Patocka J , Bocaz G
Ref : J Capill Electrophor Microchip Technol , 7 :107 , 2002
Abstract : Physostigmine (PHY) and pyridostigmine (PYR) are two important anticholinesterase compounds with several clinical uses. Recently, PHY has been investigated for the treatment of senile dementia in Alzheimer's disease. However, both PHY and PYR have gained importance as antidotes for anticholinergic drugs. In military medicine, PYR is used as a prophylactic against nerve gas poisoning and was used in Saudi Arabia during the Gulf War in 1991. A new capillary zone electrophoresis (CZE) method for the rapid determination of PHY and PYR in pharmaceutical preparations has been developed. An untreated fused-silica capillary tube (75 microm i.d., 44 cm total length, 36.5 cm length to the detector) was employed with detection at 200 and 270 nm for PHY and PYR, respectively. The optimal separation conditions for PHY were: 50 mM boric acid-HCl buffer (pH 3.25) with 30 mM NaClO4, electrokinetic injection for 5 sec at -5 kV, temperature 25 degrees C, and separation voltage 15 kV. The optimal separation conditions for PYR were: 20 mM phosphate buffer (pH 7), electrokinetic injection for 20 sec at -10 kV, temperature 25 degrees C, and separation voltage 15 kV. The limits of detection (LOD, S/N = 3) were 70 and 60 ppb for PHY and PYR, respectively. The method can be used for the monitoring of possible main degradation products in tablets of military antidote formulations.
ESTHER : Havel_2002_J.Capill.Electrophor.Microchip.Technol_7_107
PubMedSearch : Havel_2002_J.Capill.Electrophor.Microchip.Technol_7_107
PubMedID: 12546159

Title : Capillary zone electrophoresis determination of galanthamine in biological fluids and pharmaceutical preparatives: experimental design and artificial neural network optimization - Pokorna_1999_Electrophoresis_20_1993
Author(s) : Pokorna L , Revilla A , Havel J , Patocka J
Ref : Electrophoresis , 20 :1993 , 1999
Abstract : Galanthamine is a third-generation cholinesterase inhibitor used against Alzheimer's disease. New analytical methods for the determination of galanthamine in pharmaceutical preparatives and biological fluids, such as urine and serum, were developed. An experimental design and artificial neural network approach were used for method optimization. Thirty-five ppb of galanthamine were determined in serum samples (with addition of 10 mM magnesium chloride and using solid-phase preconcentration).
ESTHER : Pokorna_1999_Electrophoresis_20_1993
PubMedSearch : Pokorna_1999_Electrophoresis_20_1993
PubMedID: 10451107

Title : Capillary zone electrophoretic determination of some drugs against Alzheimer's disease - Vargas_1998_J.Chromatogr.A_802_121
Author(s) : Vargas MG , Havel J , Patocka J
Ref : Journal of Chromatography A , 802 :121 , 1998
Abstract : A new capillary zone electrophoresis (CZE) method for the determination of tacrine (THA), 7-methoxytacrine (7-MTHA) and their basic metabolites (THAm, 7-MTHAm) in pharmaceutical and biological samples (urine and serum) was developed. Separation of all compounds by CZE was carried out using a 46.6 cm untreated fused-silica capillary applying 20 kV separation voltage using 50 mM phosphate buffer of pH 2.8 for THA and THAm and of pH 7.8 for 7-MTHA and 7-MTHAm as background electrolyte (BGE). Detection was carried out at 240 nm (THA and THAm) and 248 nm (7-MTHA and 7-MTHAm). THA and THAm were separated in less than 4 min while 7-MTHA and 7-MTHAm were separated in less than 7 min. The detection limits (SIN = 3) obtained were 3 ppb for THA and 4 ppb for 7-MTHA in aqueous solutions; 50 ppb for THA and 47 ppb for 7-MTHA for the determination in urine (diluted 1:10); 52 ppb for THA and 56 ppb for 7-MTHA, in deproteinized serum samples. The methods are suitable for therapeutic drug monitoring of the drugs.
ESTHER : Vargas_1998_J.Chromatogr.A_802_121
PubMedSearch : Vargas_1998_J.Chromatogr.A_802_121
PubMedID: 9588015

Title : Determination of the anti-Alzheimer's disease drugs tacrine, 7-methoxytacrine, and its metabolites by capillary zone electrophoresis -
Author(s) : Vargas MG , Havel J , Patocka J
Ref : Am Clin Lab , 17 :22 , 1998
PubMedID: 10181004