Moran P

References (2)

Title : A process for extracellular thermostable lipase production by a novel Bacillus thermoamylovorans strain - Deive_2012_Bioprocess.Biosyst.Eng_35_931
Author(s) : Deive FJ , Alvarez MS , Moran P , Sanroman MA , Longo MA
Ref : Bioprocess Biosyst Eng , 35 :931 , 2012
Abstract : A lipolytic enzyme-producing thermophilic microorganism, recently isolated from a hot spring in Galicia (North Western Spain), has been investigated. First, the strain was genetically identified and tentatively named Bacillus thermoamylovorans CH6B. It produced significant levels (around 450 U/L) of extracellular lipolytic activity in shake flask cultures, and the most suitable conditions for this biological process were found at temperatures between 50 and 55 degrees C, and an initial pH value around 7.0. Next, a preliminary scaling up of the process was carried out in a 5-L stirred tank bioreactor, and it was concluded that operation at agitation and aeration rates of 300 rpm and 0.33 vvm, respectively, were advisable. In both type of cultures, the results were successfully fitted to logistic equations, and the relationship between lipase production and cell growth was investigated. Furthermore, some relevant properties of the crude lipolytic enzyme extracts were assessed. The crude biocatalyst preferentially hydrolysed p-nitrophenyl esters of medium and long-chain fatty acids. Thermal stability in aqueous solution of the produced enzyme was also promising, and the deactivation profiles were fitted to a series-type deactivation model.
ESTHER : Deive_2012_Bioprocess.Biosyst.Eng_35_931
PubMedSearch : Deive_2012_Bioprocess.Biosyst.Eng_35_931
PubMedID: 22237683

Title : Poster: Synthesis and biological evaluation of MDL 74,019DG, a centrally active M1 versus M2 selective muscarinic receptor antagonist -
Author(s) : Van Hijfte L , Zerr V , Hibert M , Marciniak G , Moser P , Moran P , Richards MH , van Giersbergen PL
Ref : Life Sciences , 56(11-12) :1013 , 1995