Paredes-Sanchez FA

References (2)

Title : Insecticidal Activity of Organic Extracts of Solidago graminifolia and Its Main Metabolites (Quercetin and Chlorogenic Acid) against Spodoptera frugiperda: An In Vitro and In Silico Approach - Herrera-Mayorga_2022_Molecules_27_
Author(s) : Herrera-Mayorga V , Guerrero-Sanchez JA , Mendez-Alvarez D , Paredes-Sanchez FA , Rodriguez-Duran LV , Nino-Garcia N , Paz-Gonzalez AD , Rivera G
Ref : Molecules , 27 : , 2022
Abstract : Spodoptera frugiperda (S. frugiperda) remains a global primary pest of maize. Therefore, new options to combat this pest are necessary. In this study, the insecticidal activity of three crude foliar extracts (ethanol, dichloromethane, and hexane) and their main secondary metabolites (quercetin and chlorogenic acid) of the species Solidago graminifolia (S. graminifolia) by ingestion bioassays against S. frugiperda larvae was analyzed. Additionally, the extracts were phytochemically elucidated by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis. Finally, an in silico study of the potential interaction of quercetin on S. frugiperda acetylcholinesterase was performed. Organic extracts were obtained in the range from 5 to 33%. The ethanolic extract caused higher mortality (81%) with a half-maximal lethal concentration (LC(50)) of 0.496 mg/mL. Flavonoid secondary metabolites such as hyperoside, quercetin, isoquercetin, kaempferol, and avicularin and some phenolic acids such as chlorogenic acid, solidagoic acid, gallic acid, hexoside, and rosmarinic acid were identified. In particular, quercetin had an LC(50) of 0.157 mg/mL, and chlorogenic acid did not have insecticidal activity but showed an antagonistic effect on quercetin. The molecular docking analysis of quercetin on the active site of S. frugiperda acetylcholinesterase showed a -5.4 kcal/mol binding energy value, lower than acetylcholine and chlorpyrifos (-4.45 and -4.46 kcal/mol, respectively). Additionally, the interactions profile showed that quercetin had Pi-Pi interactions with amino acids W198, Y235, and H553 on the active site.
ESTHER : Herrera-Mayorga_2022_Molecules_27_
PubMedSearch : Herrera-Mayorga_2022_Molecules_27_
PubMedID: 35630802

Title : Identification of Snp's in the Ace-1 Gene of Spodoptera frugiperda Associated with Resistance to Organophosphorus Insecticides, - Herrera-Mayorga_2018_Southwest.Entomol_43_855
Author(s) : Herrera-Mayorga EV , Bello-Ruiz DG , Paredes-Sanchez FA , Segovia-Tagle V , Garcia-Aguirre KK , Lara-Ramirez EE , Rivera G
Ref : Southwestern Entomologist , 43 :855 , 2018
Abstract : Fall armyworm, Spodoptera frugiperda (J.E. Smith), is an important pest that damages agriculture worldwide. Organophosphorus insecticides are used for its control; however, intensive and inadequate use of insecticides has selected for resistance. The ace-1 gene confers cross resistance to organophosphorus and carbamate insecticides in cholinergic synapses. SNPs associated with resistance to organophosphorus insecticide were searched using resequencing of the ace-1 gene in populations of S. frugiperda from eight municipalities at Zacatecas, Mexico: Calera, Fresnillo, General R. Murguia, Loreto, Ojocaliente, Tepechitlan, Tlaltenango, and Villanueva. The ace-1 gene was amplified by PCR and bidirectionally sequenced in third and fifth larval instars. Results indicated the presence of the g-301 mutation in the population at Loreto, and the g-565 mutation in populations at Calera, Ojocaliente, Tepechitlan, and Villanueva (50% of the populations) with 25% homozygous and 25% heterozygous. The g-380 mutation was not observed at any town. New variations in positions g-396, g-498, and g-768 also were found. Using association analysis with a dendrogram, four groups of populations were two homozygous resistant and two homozygous susceptible. The results suggested an extensive analysis of resistance of populations in the State of Zacatecas and in Mexico to establish better strategies for controlling the pest.
ESTHER : Herrera-Mayorga_2018_Southwest.Entomol_43_855
PubMedSearch : Herrera-Mayorga_2018_Southwest.Entomol_43_855
Gene_locus related to this paper: spolt-ACHE1