Sarkar R

References (3)

Title : Serum creatine phosphokinase: a probable marker of severity in organophosphorus poisoning - Bhattacharyya_2011_Toxicol.Int_18_117
Author(s) : Bhattacharyya K , Phaujdar S , Sarkar R , Mullick OS
Ref : Toxicol Int , 18 :117 , 2011
Abstract : Organophosphorus (OP), the commonest agent for poisoning in India due to its easy availability, acts by inhibiting acetylcholinesterase at muscarinic and nicotinic receptors. Erythrocyte cholinesterase (EchE) and plasma cholinesterase (PchE) are reduced in OP poisoning, but their estimation is costly and not regularly performed. There are emerging options for new cheaper biochemical markers in relation to OP poisoning. Serum level of creatine phosphokinase (CPK) is often found to be elevated in OP poisoning. This study was conducted to see if CPK may be used as an alternative of cholinesterase levels in blood to assess the severity of OP poisoning. This was a prospective and observational study. Sixty-three patients of OP poisoning without any prior treatment, presenting within 6 hours, were selected and their clinical severity was categorized according to Peradeniya organophosphorus poisoning (POP) scale. Level of serum CPK, blood EchE and pH were measured following admission, and total dose of atropine (mg) until the final clinical outcome (complete recovery or death) was calculated. Student's t-test and Pearson's correlation coefficient was used for the assessment of statistical significance. According to POP scale, clinical severity was mild (score 0-3) in 17 (27%), moderate (score 4-7) in 32 (50.8%) and severe (score 8-11) in 14 (22.2%) patients. Serum CPK, EchE level, blood pH and total atropine dose strongly correlated with clinical severity. Our study recommends serum CPK as an alternative marker.
ESTHER : Bhattacharyya_2011_Toxicol.Int_18_117
PubMedSearch : Bhattacharyya_2011_Toxicol.Int_18_117
PubMedID: 21976816

Title : Effects of an organophosphate pesticide, quinalphos, on the hypothalamo-pituitary-gonadal axis in adult male rats - Sarkar_2000_J.Reprod.Fertil_118_29
Author(s) : Sarkar R , Mohanakumar KP , Chowdhury M
Ref : J Reprod Fertil , 118 :29 , 2000
Abstract : The effects of chronic sub-lethal doses (7-14 mg kg-1 a day for 15 days) of quinalphos were evaluated in adult male rats for changes in testicular morphology, circulatory concentrations of hormones (LH, FSH, prolactin and testosterone), activities of acetylcholinesterase (AChE) and angiotensin converting enzyme (ACE) as well as metabolism of biogenic amines (dopamine, noradrenaline and 5-hydroxytryptamine (5-HT)) in the hypothalamus and pituitary. Hormones were assayed by radioimmunoassay or chemiluminescent immunoassay (testosterone). The enzymes were estimated after spectrophotometry and the biogenic amines by HPLC-electrochemistry. Sub-lethal chronic administration of quinalphos resulted in: decreased testicular mass and AChE activity in central as well as peripheral organs; increased serum LH, FSH, prolactin and testosterone concentrations; decreased pituitary or increased testicular ACE activity; severe disruption of spermatogenesis with increasing doses of pesticide; and no significant effects on dopamine, noradrenaline or 5-HT concentrations in the hypothalamus or pituitary. Administration of oestradiol (50 micrograms per rat a day) during pesticide treatment resulted in: a significant decrease in the mass of the testis and accessory sex organs; decreases in serum LH, FSH, testosterone concentrations; an increase in prolactin concentration; and a decrease in dopamine or an increase in noradrenaline and 5-HT in the hypothalamus or pituitary. Oestradiol had a marked effect: in pesticide-treated animals, the pesticide effects were significantly reversed. This indicates that in pesticide toxicity, the hypothalamo-pituitary-gonadal axis is operational. Since many of the observed pesticide effects could be inhibited by oestradiol, it is suggested that the pesticide acts directly on the gonadotrophins. In conclusion, quinalphos decreases fertility in adult male rats by affecting the pituitary gonadotrophins.
ESTHER : Sarkar_2000_J.Reprod.Fertil_118_29
PubMedSearch : Sarkar_2000_J.Reprod.Fertil_118_29
PubMedID: 10793623

Title : Influence of methyl parathion on gametogenic and acetylcholinesterase activity in the testis of whitethroated munia (Lonchura malabarica) - Maitra_1996_Arch.Environ.Contam.Toxicol_30_384
Author(s) : Maitra SK , Sarkar R
Ref : Archives of Environmental Contamination & Toxicology , 30 :384 , 1996
Abstract : Adult male whitethroated munias, Lonchura malabarica (Aves; Passeriformes), were orally administered with methyl parathion (O, O-dimethyl O-(4-nitrophenyl) phosphorothioate), an extensively used organophosphate pesticide, in graded sublethal dose (5 micro g-, or 10 micro g-, or 20 micro g/100 g body wt/day) for variable durations (1-, 5-, or 10 day/s) during their peak reproductive activities in an annual gonadal cycle. No subtle changes in the feeding behavior, mobility, and body weight were noted between the control and different groups of pesticide-fed birds. As a result of the treatment, the paired testicular weight became reduced significantly only after 10 days at 10 micro g and 20 micro g dose levels, but significant decrease in the number of tubules containing healthy germ cells occurred even after single administration of methyl parathion (MP) at the lowest dose (5 micro g/100 g). With the increase in dose and progress of treatment, the number of tubules with healthy germ cells became gradually decreased. The activity of acetylcholinesterase (AChE) in both the brain and testes of MP-treated birds was inhibited in a dose and duration dependent manner. A significant negative correlation was observed between the number of tubules containing degenerated germ cells in the testis and the AChE activity in both the brain and testes of MP-administered birds. However, no remarkable changes in the cytomorphological features, including the nuclear diameter of Leydig cells, were noted in any testis of the pesticide-treated munias. The results of the present investigation suggest that methyl parathion ingestion is harmful to male gametogenic functions in the studied passeriform bird, and the given pesticide may exert its antigonadal effect by impairing cholinergic functions of the brain and/or testes.
ESTHER : Maitra_1996_Arch.Environ.Contam.Toxicol_30_384
PubMedSearch : Maitra_1996_Arch.Environ.Contam.Toxicol_30_384
PubMedID: 885496