Savas CP

References (2)

Title : Transition metal cation inhibition of Mycobacterium tuberculosis esterase RV0045C - Bowles_2021_Protein.Sci__
Author(s) : Bowles IE , Pool EH , Lancaster BS , Lawson EK , Savas CP , Kartje ZJ , Severinac L , Cho DH , Macbeth MR , Johnson RJ , Hoops GC
Ref : Protein Science , : , 2021
Abstract : Mycobacterium tuberculosis virulence is highly metal-dependent with metal availability modulating the shift from the dormant to active states of M. tuberculosis infection. Rv0045c from M. tuberculosis is a proposed metabolic serine hydrolase whose folded stability is dependent on divalent metal concentration. Herein, we measured the divalent metal inhibition profile of the enzymatic activity of Rv0045c and found specific divalent transition metal cations (Cu(2+) <= Zn(2+) > Ni(2+) > Co(2+) ) strongly inhibited its enzymatic activity. The metal cations bind allosterically, largely affecting values for k(cat) rather than K(M) . Removal of the artificial N-terminal 6xHis-tag did not change the metal-dependent inhibition, indicating that the allosteric inhibition site is native to Rv0045c. To isolate the site of this allosteric regulation in Rv0045c, the structures of Rv0045c were determined at 1.8 A and 2.0 A resolution in the presence and absence of Zn(2+) with each structure containing a previously unresolved dynamic loop spanning the binding pocket. Through the combination of structural analysis with and without zinc and targeted mutagenesis, this metal-dependent inhibition was traced to multiple chelating residues (H202A/E204A) on a flexible loop, suggesting dynamic allosteric regulation of Rv0045c by divalent metals. Although serine hydrolases like Rv0045c are a large and diverse enzyme superfamily, this is the first structural confirmation of allosteric regulation of their enzymatic activity by divalent metals. This article is protected by copyright. All rights reserved.
ESTHER : Bowles_2021_Protein.Sci__
PubMedSearch : Bowles_2021_Protein.Sci__
PubMedID: 33914998
Gene_locus related to this paper: myctu-RV0045C

Title : Distinct Substrate Selectivity of a Metabolic Hydrolase from Mycobacterium tuberculosis - Lukowski_2014_Biochemistry_53_7386
Author(s) : Lukowski JK , Savas CP , Gehring AM , McKary MG , Adkins CT , Lavis LD , Hoops GC , Johnson RJ
Ref : Biochemistry , 53 :7386 , 2014
Abstract : The transition between dormant and active Mycobacterium tuberculosis infection requires reorganization of its lipid metabolism and activation of a battery of serine hydrolase enzymes. Among these serine hydrolases, Rv0045c is a mycobacterial-specific serine hydrolase with limited sequence homology outside mycobacteria but structural homology to divergent bacterial hydrolase families. Herein, we determined the global substrate specificity of Rv0045c against a library of fluorogenic hydrolase substrates, constructed a combined experimental and computational model for its binding pocket, and performed comprehensive substitutional analysis to develop a structural map of its binding pocket. Rv0045c showed strong substrate selectivity toward short, straight chain alkyl esters with the highest activity toward four atom chains. This strong substrate preference was maintained through the combined action of residues in a flexible loop connecting the cap and alpha/beta hydrolase domains and in residues close to the catalytic triad. Two residues bracketing the substrate-binding pocket (Gly90 and His187) were essential to maintaining the narrow substrate selectivity of Rv0045c toward various acyl ester substituents, as independent conversion of these residues significantly increased its catalytic activity and broadened its substrate specificity. Focused saturation mutagenesis of position 187 implicated this residue, as the differentiation point between the substrate specificity of Rv0045c and the structurally homologous ybfF hydrolase family. Insertion of the analogous tyrosine residue from ybfF hydrolases into Rv0045c increased the catalytic activity of Rv0045 by over 20-fold toward diverse ester substrates. The unique binding pocket structure and selectivity of Rv0045c provide molecular indications of its biological role and evidence for expanded substrate diversity in serine hydrolases from M. tuberculosis.
ESTHER : Lukowski_2014_Biochemistry_53_7386
PubMedSearch : Lukowski_2014_Biochemistry_53_7386
PubMedID: 25354081
Gene_locus related to this paper: myctu-RV0045C