Tsuge H

References (2)

Title : Substrate selectivity of bacterial monoacylglycerol lipase based on crystal structure - Tsurumura_2014_J.Struct.Funct.Genomics_15_83
Author(s) : Tsurumura T , Tsuge H
Ref : J Struct Funct Genomics , 15 :83 , 2014
Abstract : Lipases, which are conserved from bacteria to mammals, catalyze the hydrolysis of acylglycerol to free fatty acids and glycerol. Monoacylglycerol lipase (MGL) specifically catalyzes the hydrolysis of monoacylglycerol. Although there have been numerous studies of the structure of lipases, there have been few studies of MGL. Here, we report the crystal structure of authentic MGL isolated from Bacillus sp. H257 (bMGL). The crystal diffracts to 1.96 A resolution. It belongs to space group P21212, and the unit cell parameters are a = 99.7 A, b = 106.1 A and c = 43.0 A. As in other lipases, three structural features for lipase activity are conserved in bMGL: the glycine-X-serine-X-glycine motif, catalytic triad and cap region. The structure of bMGL appears to be closed, as the cap region covers the active site entrance. The isolated bMGL hydrolyzed 2-AG, a known human MGL-specific substrate. Based on a 2-AG bound model, we discuss the substrate selectivity. The functional and structural features of bMGL provide insight how its substrate selectivity is determined and how specific inhibitors of bacterial MGL could be designed, which may be useful for development of novel antibiotics.
ESTHER : Tsurumura_2014_J.Struct.Funct.Genomics_15_83
PubMedSearch : Tsurumura_2014_J.Struct.Funct.Genomics_15_83
PubMedID: 24894647
Gene_locus related to this paper: bac25-mglp

Title : Crystallization and preliminary X-ray crystallographic studies of monoacylglycerol lipase of the moderately thermophilic Bacillus sp. H-257 - Yoneda_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1232
Author(s) : Yoneda K , Nishimura T , Katunuma N , Imamura S , Nitta K , Tsuge H
Ref : Acta Crystallographica D Biol Crystallogr , 58 :1232 , 2002
Abstract : Thermostable monoacylglycerol lipase (MGLP; EC from the moderately thermophilic Bacillus sp. H-257 has a unique substrate specificity. It hydrolyzes monoacylglycerols but does not hydrolyze di- or triacylglycerols. Crystals of the enzyme were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and benzamidine as an additive. The orthorhombic crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 43.53, b = 100.82, c = 108.17 A. The crystals diffract to at least 2.3 A resolution and a native data set has been collected to 2.6 A resolution on a CCD detector using synchrotron radiation.
ESTHER : Yoneda_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1232
PubMedSearch : Yoneda_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1232
PubMedID: 12077453
Gene_locus related to this paper: bac25-mglp