Wang HT

References (2)

Title : Lycodine type alkaloids from Lycopodiastrum casuarinoides with cytotoxic and cholinesterase inhibitory activities - Qu_2018_Fitoterapia_131_86
Author(s) : Qu SM , Shan BH , Wang HT , Wang S
Ref : Fitoterapia , 131 :86 , 2018
Abstract : A chemical investigation on the 70% EtOH extract of the aerial parts of Lycopodiastrum casuarinoides led to the isolation of six novel lycodine type alkaloids, lycocasuarines A-F (1-6). The structures of the isolated compounds were established based on 1D and 2D ((1)H(1)H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated alkaloids were tested in vitro for cytotoxic potentials against seven malignant melanoma cell lines as well as acetylcholinesterase (AChE) and butyrocholinesterase (BuChE) inhibitory activities. As a result, alkaloids 1 and 3 exhibited significant cytotoxic activities against all the tested tumor cell lines with IC50 values <10muM and the inhibitory activities for AchE (0.94+/-0.15 and 0.24+/-0.03muM, respectively) and BuchE (1.82+/-0.12 and 7.31+/-0.42muM, respectively).
ESTHER : Qu_2018_Fitoterapia_131_86
PubMedSearch : Qu_2018_Fitoterapia_131_86
PubMedID: 30352296

Title : Donepezil improves learning and memory deficits in APP\/PS1 mice by inhibition of microglial activation - Guo_2015_Neurosci_290C_530
Author(s) : Guo HB , Cheng YF , Wu JG , Wang CM , Wang HT , Zhang C , Qiu ZK , Xu JP
Ref : Neuroscience , 290C :530 , 2015
Abstract : Donepezil, a cholinesterase inhibitor, is a representative symptomatic therapy for Alzheimer's disease (AD). Recent studies have reported the anti-inflammatory effects of donepezil. However, limited studies that investigate its anti-inflammatory effect in AD have been reported. Considering the role of proinflammatory molecules and microglial activation in the pathogenesis of AD, the current study aimed to elucidate the effects of donepezil on microglial activation induced by amyloid deposition in transgenic mice. Our results showed that chronic treatment with donepezil significantly improved the cognitive function in the novel object recognition test and Morris water maze test in amyloid precursor protein (APP)/presenilin-1 (PS1) transgenic mice. We further demonstrated that these cognitive enhancements were related to the anti-inflammatory effect of donepezil. We found that donepezil could inhibit the expression of CD68, a specific marker of microglial activation, and reduce the release of proinflammatory cytokines including tumor necrosis factor-alpha and interleukin-1beta. Immunohistochemistry and Congo red co-staining revealed that congophilic amyloid and activated microglia around plaques were also reduced by donepezil treatment. Enzyme-linked immunosorbent assay (ELISA) analysis showed that donepezil decreased insoluble Abeta40/Abeta42 and soluble Abeta40 levels. Moreover, donepezil reversed the impaired expression of insulin-degrading enzyme in the hippocampus of APP/PS1 mice. Our findings indicated that donepezil improves cognitive deficits in APP/PS1 mice by a mechanism that may be associated with its inhibition of microglial activation and release of proinflammatory cytokines.
ESTHER : Guo_2015_Neurosci_290C_530
PubMedSearch : Guo_2015_Neurosci_290C_530
PubMedID: 25662507