Xin M

References (3)

Title : Plasma exchange therapy for familial chylomicronemia syndrome in infant: A case report - Han_2022_Medicine.(Baltimore)_101_e29689
Author(s) : Han L , Qiang G , Yang L , Kou R , Li Q , Xin M , Liu R , Zhang Z
Ref : Medicine (Baltimore) , 101 :e29689 , 2022
Abstract : INTRODUCTION: Familial chylomicronemia syndrome (FCS) is a rare genetic disease. FCS usually manifests by the age of 10 years, and 25% of cases of FCS occur during infancy. Here we present a case of FCS in a male infant and summarize our experiences on the diagnosis and therapy of this case. PATIENT CONCERNS: A male infant aged 1 month and 8 days had recurrent hematochezia and hyperchylomicronemia. DIAGNOSIS: FCS based on symptoms and genetic test. INTERVENTIONS: Plasma exchange therapy. OUTCOMES: His development was normal with a good spirit and satisfactory weight gain, and no hematochezia occurred again. CONCLUSION: Genetic test is important for accurate diagnosis of FCS, and we identified a new mutation of lipoprotein lipase gene c.88C>A which conformed to autosomal recessive inheritance. Plasma exchange therapy can be applied to infants with FCS with low risk and good outcomes.
ESTHER : Han_2022_Medicine.(Baltimore)_101_e29689
PubMedSearch : Han_2022_Medicine.(Baltimore)_101_e29689
PubMedID: 35960041
Gene_locus related to this paper: human-LPL

Title : The Reference Genome of the Halophytic Plant Eutrema salsugineum - Yang_2013_Front.Plant.Sci_4_46
Author(s) : Yang R , Jarvis DE , Chen H , Beilstein MA , Grimwood J , Jenkins J , Shu S , Prochnik S , Xin M , Ma C , Schmutz J , Wing RA , Mitchell-Olds T , Schumaker KS , Wang X
Ref : Front Plant Sci , 4 :46 , 2013
Abstract : Halophytes are plants that can naturally tolerate high concentrations of salt in the soil, and their tolerance to salt stress may occur through various evolutionary and molecular mechanisms. Eutrema salsugineum is a halophytic species in the Brassicaceae that can naturally tolerate multiple types of abiotic stresses that typically limit crop productivity, including extreme salinity and cold. It has been widely used as a laboratorial model for stress biology research in plants. Here, we present the reference genome sequence (241 Mb) of E. salsugineum at 8x coverage sequenced using the traditional Sanger sequencing-based approach with comparison to its close relative Arabidopsis thaliana. The E. salsugineum genome contains 26,531 protein-coding genes and 51.4% of its genome is composed of repetitive sequences that mostly reside in pericentromeric regions. Comparative analyses of the genome structures, protein-coding genes, microRNAs, stress-related pathways, and estimated translation efficiency of proteins between E. salsugineum and A. thaliana suggest that halophyte adaptation to environmental stresses may occur via a global network adjustment of multiple regulatory mechanisms. The E. salsugineum genome provides a resource to identify naturally occurring genetic alterations contributing to the adaptation of halophytic plants to salinity and that might be bioengineered in related crop species.
ESTHER : Yang_2013_Front.Plant.Sci_4_46
PubMedSearch : Yang_2013_Front.Plant.Sci_4_46
PubMedID: 23518688
Gene_locus related to this paper: theha-e4mxu0 , thesl-v4nk72 , eutsa-v4l4z1 , eutsa-v4kk46 , eutsa-v4mej3 , eutsa-v4ns11 , eutsa-v4mg02 , eutsa-v4mqm9 , eutsa-v4k1y6 , eutsa-v4lad0 , eutsa-v4nr92 , eutsa-v4kqc3 , eutsa-v4l0s2 , eutsa-v4lip3 , eutsa-v4kkg2 , eutsa-v4kvd3 , eutsa-v4m9g4 , eutsa-v4lqg2 , eutsa-v4lp36 , eutsa-v4km66 , eutsa-v4nhr8 , eutsa-v4kqx9 , eutsa-v4lv73

Title : An X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis: cloning, expression in Escherichia coli, and application for removal of N-terminal Pro-Pro from recombinant proteins - Xin_2002_Protein.Expr.Purif_24_530
Author(s) : Xin M , Li Y , Jie L , Min D , Liu J
Ref : Protein Expr Purif , 24 :530 , 2002
Abstract : A novel pepX gene was cloned from isolated DNA of Lactococcus lactis by PCR. The deduced amino acid sequence of the 89-kDa protein showed 94, 93, 65, and 44% identity with the pepX protein from Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Lactobacillus delbruecki subsp. bulgaricus, and Lactobacillus helveticus, respectively, and contained a serine protease G-K-S-Y-L-G consensus motif. The pepX gene has been cloned into pET17b and was expressed at a high level in Escherichia coli BL21 (DE3) LysS. PepX was purified to approximate homogeneity with ammonium sulfate precipitation and DEAE Sephadex A-50 chromatography. Optimal pepX activity was observed at pH 8.0 and 37 degrees C. According to SDS-PAGE analysis, pepX has a molecular mass of approximately 89 kDa. The peptidase can remove completely the unwanted X-Pro from the N-terminal of the target protein, releasing the naturally active protein and peptide, revealing a prospective application of pepX in large-scale production of pharmaceutical protein and peptide products.
ESTHER : Xin_2002_Protein.Expr.Purif_24_530
PubMedSearch : Xin_2002_Protein.Expr.Purif_24_530
PubMedID: 11922771