The bottromycin epimerase BotH defines a group of atypical alpha/beta-hydrolase-fold enzymes. BotH is a peptide epimerase responsible for the post-translational epimerization of L-Asp to D-Asp during bottromycin biosynthesis. D-amino acids endow peptides with diverse, desirable properties, but the post-translational and site-specific epimerization of L-amino acids into their D-counterparts is rare and chemically challenging. The activity does not rely on a catalytic triad. No conservation of Ser (replaced by Phe) nor His or Asp. The residues Phe110 and Val41 seem important in hydrogen bonding to substrate
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Structures (5)
Genes Proteins in BotH family (10)
No fragment of genes
Structures in BotH family (5)Substrates in BotH family (4)
No Inhibitor
Title : The bottromycin epimerase BotH defines a group of atypical alpha\/beta-hydrolase-fold enzymes - Sikandar_2020_Nat.Chem.Biol_16_1013 |
Author(s) : Sikandar A , Franz L , Adam S , Santos-Aberturas J , Horbal L , Luzhetskyy A , Truman AW , Kalinina OV , Koehnke J |
Ref : Nat Chemical Biology , 16 :1013 , 2020 |
Abstract : Sikandar_2020_Nat.Chem.Biol_16_1013 |
ESTHER : Sikandar_2020_Nat.Chem.Biol_16_1013 |
PubMedSearch : Sikandar_2020_Nat.Chem.Biol_16_1013 |
PubMedID: 32601484 |
Gene_locus related to this paper: 9actn-k4mhv9 |