uncultured Pseudomonadales: Pseudomonas sp. PETase MG8
From metagenome: MCA23DSaliva (human oral metagenome). MG8, from the human saliva metagenome has robust PET plastic degradation activities under different temperature and salinity conditions, outperforming several naturally occurring and engineered hydrolases in degrading PET. By encoding 2,3-diaminopropionic acid (DAP) in place of the catalytic serine of MG8, this non-canonical amino acid was used to covalently trap the acyl-enzyme intermediate of MG8. So this PET-degrading enzyme was converted into a covalent binder of PET. MG8(DAP) covalently captures instead of degrading PET
Family : Polyesterase-lipase-cutinase
Block : L
Position in NCBI Life Tree : uncultured Pyrenomonadales
MKALTFSKSF LSAIAAGALM LSASAMANNP PPPDDPGAPS PYQRGPDPTV SFVEASRGNY RVATSNVSSL VSGFGGGTIH YPSNATGTMG AIVVIPGYVS GEGSIDWWGP KLASYGFVVM TIGTNSGFDQ PPSRARQINN ALDYLVEQNT RTGSPVRGMI DPSRLGVIGW SMGGGGTIRV AGEGRIKAAI PLAPWDTSSI PSRGVQAPTL IFACQSDVVA PVRSHASPFY NALPGSISKA FVNLNNGNHF CANGGSSFGR YDAALGRLGV SWMKRFLDED RRYSQFLCGP NHTGDRQITE YRGNCPY
| Title : Discovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET - Eiamthong_2022_Angew.Chem.Int.Ed.Engl_15_e202203061 |
| Author(s) : Eiamthong B , Meesawat P , Wongsatit T , Jitdee J , Sangsri R , Patchsung M , Aphicho K , Suraritdechachai S , Huguenin-Dezot N , Tang S , Suginta W , Paosawatyanyong B , Babu MM , Chin JW , Pakotiprapha D , Bhanthumnavin W , Uttamapinant C |
| Ref : Angew Chem Int Ed Engl , :e202203061 , 2022 |
| Abstract : Eiamthong_2022_Angew.Chem.Int.Ed.Engl_15_e202203061 |
| ESTHER : Eiamthong_2022_Angew.Chem.Int.Ed.Engl_15_e202203061 |
| PubMedSearch : Eiamthong_2022_Angew.Chem.Int.Ed.Engl_15_e202203061 |
| PubMedID: 35656865 |
| Gene_locus related to this paper: 9gamm-PETaseMG1 , 9gamm-PETaseMG2 , 9gamm-PETaseMG3 , 9gamm-PETaseMG4 , 9gamm-PETaseMG5 , 9gamm-PETaseMG6 , 9gamm-PETaseMG7 , 9gamm-PETaseMG8 , 9pseu-PETaseMG9 , 9actn-PETaseMG10 |