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halsp-est

Halobacterium sp. NRC-1 est rHsEst halophilic esterase

Comment

HsEst was stable and active in NaCl concentrations as high as 5M. p-nitrophenyl valerate as substrate (Km = 78 microM, kcat = 0.67 s1). rHsEst exhibited great stability to most metal ions tested and some solvents (diethyl ether, n-hexane, n-heptane)

Relationship

Family : 6_AlphaBeta_hydrolase

Block : X

Position in NCBI Life Tree : Halobacterium sp. NRC-1

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Archaea N E > Euryarchaeota N E > Halobacteria N E > Halobacteriales N E > Halobacteriaceae N E > Halobacterium N E > Halobacterium salinarum N E > Halobacterium salinarum NRC-1 N E

Molecular evidence

No mutation

No structure

No kinetic

No disease

No substrate

No inhibitor

Database

Sequence

Peptide

MPLVALAATM ETLAHHGRST AYRVRGSADA PTVLFVHGSG GTHAVWKAQL ARLADTYRVA ALDLPGHGDS DDIETDPGPE TLDAYAADVR AVAADVGASV LVGNSLGGAV VLTAVLDGGV EPDAVVLAGC GATLAVADSL RAWLAGDGPG FERALAFLHD SGRLFHDPDD RTLALSKDAM RDCGRAVVNR DFRSCHSFDV RTRLSEIDSP VFALTGDHDH LTPPSAHEFL AEHVQTGGWT TLPDAAHLSM LETPDQFNDA LDAFLSDPAL VARR

References (2)

Title : Cloning, Expression, Characterization and Immobilization of a Recombinant Carboxylesterase from the Halophilic Archaeon, Halobacterium salinarum NCR-1 - Ortega-de la Rosa_2024_Biomolecules_14_534
Author(s) : Ortega-de la Rosa ND , Romero-Borbon E , Rodriguez JA , Camacho-Ruiz A , Cordova J
Ref : Biomolecules , 14 :534 , 2024
Abstract :
PubMedSearch : Ortega-de la Rosa_2024_Biomolecules_14_534
PubMedID: 38785941
Gene_locus related to this paper: halsp-est

Title : Genome sequence of Halobacterium species NRC-1 - Ng_2000_Proc.Natl.Acad.Sci.U.S.A_97_12176
Author(s) : Ng WV , Kennedy SP , Mahairas GG , Berquist B , Pan M , Shukla HD , Lasky SR , Baliga NS , Thorsson V , Sbrogna J , Swartzell S , Weir D , Hall J , Dahl TA , Welti R , Goo YA , Leithauser B , Keller K , Cruz R , Danson MJ , Hough DW , Maddocks DG , Jablonski PE , Krebs MP , Angevine CM , Dale H , Isenbarger TA , Peck RF , Pohlschroder M , Spudich JL , Jung KW , Alam M , Freitas T , Hou S , Daniels CJ , Dennis PP , Omer AD , Ebhardt H , Lowe TM , Liang P , Riley M , Hood L , DasSarma S
Ref : Proc Natl Acad Sci U S A , 97 :12176 , 2000
Abstract :
PubMedSearch : Ng_2000_Proc.Natl.Acad.Sci.U.S.A_97_12176
PubMedID: 11016950
Gene_locus related to this paper: halsp-est , halsp-metx , halsp-VNG0492H , halsp-VNG0675C , halsp-VNG1418C , halsp-VNG1833C , halsp-Vng6296c , halsp-YUXL