Gene Locus : human-PLA2G7
Mode of mutation : Site directed mutagenesis
Disease :
Summary :
AAA Change :
Allelic Variant :
Risk Factor :
Inhibitor :
Structure :
Disease by interaction :
Interact Gene Locus :
Xenobiotic sensitivity :
Modification :
Torpedo_number : No torpedo number
Kinetic Parameter : No kinetic parameter
News : No news
Comment :
Tentative to engineer plasma platelet-activating factor acetylhydrolase by amino acid substitution into OP-hydrolyzing variants (bioscavengers). L153H, and F322H have essentially no hydrolytic activity against the nerve agents tested. In contrast, the W298H mutant displayed novel somanase activity with a kcat of 5min-1 and a KM of 590muM at pH7.5.
| Title : A rationally designed mutant of plasma platelet-activating factor acetylhydrolase hydrolyzes the organophosphorus nerve agent soman - Kirby_2015_Biochim.Biophys.Acta_1854_1809 |
| Author(s) : Kirby SD , Norris J , Sweeney R , Bahnson BJ , Cerasoli DM |
| Ref : Biochimica & Biophysica Acta , 1854 :1809 , 2015 |
| Abstract : |
| PubMedSearch : Kirby_2015_Biochim.Biophys.Acta_1854_1809 |
| PubMedID: 26343853 |
| Gene_locus related to this paper: human-PLA2G7 |