F322H_human-PLA2G7

General

Gene Locus : human-PLA2G7

Mode of mutation : Site directed mutagenesis

Disease :

Summary :

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification :

Torpedo_number : No torpedo number

Kinetic Parameter : No kinetic parameter

News : No news

Comment :
Tentative to engineer plasma platelet-activating factor acetylhydrolase by amino acid substitution into OP-hydrolyzing variants (bioscavengers). L153H, and F322H have essentially no hydrolytic activity against the nerve agents tested. In contrast, the W298H mutant displayed novel somanase activity with a kcat of 5min-1 and a KM of 590muM at pH7.5.

References (1)

Title : A rationally designed mutant of plasma platelet-activating factor acetylhydrolase hydrolyzes the organophosphorus nerve agent soman - Kirby_2015_Biochim.Biophys.Acta_1854_1809
Author(s) : Kirby SD , Norris J , Sweeney R , Bahnson BJ , Cerasoli DM
Ref : Biochimica & Biophysica Acta , 1854 :1809 , 2015
Abstract :
PubMedSearch : Kirby_2015_Biochim.Biophys.Acta_1854_1809
PubMedID: 26343853
Gene_locus related to this paper: human-PLA2G7