Abdullah_2025_Biomolecules_15_

Tordylium trachycarpum Boiss. (Apiaceae) has long been used by traditional healers in the Kurdistan Region of Iraq to alleviate gastrointestinal disorders and oral inflammation; however, its phytochemical composition and pharmacological properties remain scientifically unverified. In this study, we report the first phytochemical profiling and plant-assisted synthesis of copper nanoparticles (CuNPs) using the methanolic extract of T. trachycarpum as a natural reducing and stabilizing agent. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, FTIR spectroscopy, X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Energy-Dispersive X-ray Spectroscopy (EDS) analyses, confirming their nanoscale formation, crystallinity, and elemental composition. Gas chromatography-mass spectrometry (GC-MS) identified 22 bioactive metabolites, with methoxsalen (30.91%), triphenylphosphine oxide (12.54%), desulphosinigrin (10.79%), isopimpinellin (6.72%), and alpha-glyceryl linolenate (6.39%) as the predominant constituents. Both the crude extract and the biosynthesized CuNPs were evaluated for their antimicrobial, antioxidant, and enzyme inhibitory activities. The CuNPs displayed enhanced antimicrobial potency, with MIC values of 250 microg/mL against Klebsiella pneumoniae and Candida albicans, and 500 microg/mL against Pseudomonas aeruginosa and Staphylococcus epidermidis. They also exhibited superior antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), cupric ion reducing antioxidant capacity (CUPRAC), and metal chelating activity (MCA) assays, along with moderate inhibition of key metabolic and neurological enzymes, including acetylcholinesterase and tyrosinase. These findings highlight T. trachycarpum as a promising phytochemical source for sustainable nanoparticle synthesis and reveal the multifunctional potential of biosynthesized CuNPs as antioxidant and antimicrobial agents with prospective applications in drug discovery and nanomedicine.