Adcock_1981_Pest.Sci_12_645

A sensitive and specific method for the determination of the bendiocarb metabolite 2, 2-dimethylbenzo-1, 3-dioxol-4-ol (I) in human urine has been developed. The method can be used to estimate the amount of bendiocarb absorbed by personnel if they become exposed to the insecticide during manufacture and spraying. Urine is collected for 16 h after the period of exposure to bendiocarb and the total volume of urine voided is measured. The urinary metabolite I occurs in conjugated form and is released by enzymic hydrolysis. The free I is extracted from the urine and analysed by gas chromatography/mass fragmentography. Deuterated I is used as an internal standard and is added to the urine sample prior to the hydrolysis procedure. Determination is possible at levels of 1 ug of I ml-1 of urine. Knowing the total volume of urine voided, the amount of bendiocarb absorbed can be calculated. The method can determine the absorption of 1 mg of bendiocarb and will detect absorption below that amount.