| Title : Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors - Adem_1988_Biochim.Biophys.Acta_968_340 |
| Author(s) : Adem A , Asblom A , Johansson G , Mbugua PM , Karlsson E |
| Ref : Biochimica & Biophysica Acta , 968 :340 , 1988 |
|
Abstract :
Two protein toxins that displace the muscarinic antagonist quinuclidinyl benzilate from rat cortex synaptosomal membranes have been isolated from the green mamba (Dendroaspis angusticeps) venom by gel filtration on sephadex G-50, chromatography on the ion-exchangers Bio-Rex 70 and Sulphopropyl-Sephadex C-25 and reversed-phase HPLC. Toxin 1 has 64 amino acids and four disulfides and a formula weight of 7200 and the corresponding values for toxin 2 are 63, 4 and 6840, respectively. Ultracentrifugation gave a molecular weight of 6900 for toxin 1 and 6700 for toxin 2, Quinuclidinyl benzilate that binds to all types of muscarinic cholinergic receptor was displaced to about 50% by both toxins. This partial displacement indicates that the toxins might be specific for one subtype of receptor. |
| PubMedSearch : Adem_1988_Biochim.Biophys.Acta_968_340 |
| PubMedID: 3345316 |
Adem A, Asblom A, Johansson G, Mbugua PM, Karlsson E (1988)
Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors
Biochimica & Biophysica Acta
968 :340
Adem A, Asblom A, Johansson G, Mbugua PM, Karlsson E (1988)
Biochimica & Biophysica Acta
968 :340