Agatonovic-Kustrin_2023_J.Pharm.Biomed.Anal_227_115308

Reference

Title : Effect directed analysis of bioactive compounds in leaf extracts from two Salvia species by High-performance thin-layer chromatography - Agatonovic-Kustrin_2023_J.Pharm.Biomed.Anal_227_115308
Author(s) : Agatonovic-Kustrin S , Wong S , Dolzhenko AV , Gegechkori V , Ku H , Tan WK , Morton DW
Ref : J Pharm Biomed Anal , 227 :115308 , 2023
Abstract :

Extracts of two Salvia species, Salvia apiana (white sage) and Salvia officinalis (common sage) were screened for phytoconstituents with the ability to act as antidiabetic, cognitive enhancing, or antimicrobial agents, by hyphenation of high-performance thin-layer chromatography with enzymatic and microbial effect directed assays. Two bioactive zones with alpha-amylase inhibition (zone 1 and zone 2), 3 zones for acetylcholinesterase inhibition (zones 3, 4 and 5), and two zones for antimicrobial activity (zones 4 and 5) were detected. The compounds from the five bioactive zones were initially identified by coelution with standards and comparing the R(F) values of standards to the bioautograms. Identity was confirmed with ATR-FTIR spectra of the isolated compounds from the bioactive zones. A significantly higher alpha-amylase and acetylcholinesterase inhibition of S. apiana leaf extract was associated with a higher flavonoid and diterpenoid content. Fermented S. officinalis extract exhibited a significantly higher ability to inhibit alpha-amylase compared to other non-fermented extracts from this species, due to increased extraction of flavonoids. The ATR-FTIR spectra of 2 zones with alpha-amylase inhibition, indicated that flavonoids and phenolic acids were responsible for alpha-amylase inhibition. Multiple zones of acetylcholinesterase inhibition were related to the presence of phenolic abietane diterpenoids and triterpenoid acids. The presence of abietane diterpenoids and triterpenoid acids was also found responsible for the mild antimicrobial activity. Flash chromatography was used to isolate sufficient amounts of bioactive compounds for further characterisation via NMR and MS spectroscopy. Five compounds were assigned to the zones where bioactivity was observed: cirsimaritin (zone 1), a caffeic acid polymer (zone 2), 16-hydroxyrosmanol (zone 3), 16-hydroxycarnosic acid (zone 4), oleanolic and ursolic acids (zone 5).

PubMedSearch : Agatonovic-Kustrin_2023_J.Pharm.Biomed.Anal_227_115308
PubMedID: 36827737

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Citations formats

Agatonovic-Kustrin S, Wong S, Dolzhenko AV, Gegechkori V, Ku H, Tan WK, Morton DW (2023)
Effect directed analysis of bioactive compounds in leaf extracts from two Salvia species by High-performance thin-layer chromatography
J Pharm Biomed Anal 227 :115308

Agatonovic-Kustrin S, Wong S, Dolzhenko AV, Gegechkori V, Ku H, Tan WK, Morton DW (2023)
J Pharm Biomed Anal 227 :115308