Akbaba_2026_Chem.Biodivers_23_e03588

Comparative data on different parts of Acca sellowiana and their enzyme-inhibitory mechanisms remain limited. This study evaluated flower, fruit, leaf, and branch extracts using bioactivity-guided LC-MS/MS and molecular docking to clarify their antioxidant and antidiabetic potential. Total phenolic, flavonoid, and tannin contents were measured, antioxidant activity was assessed by DPPH and ABTS assays, and inhibition of alpha-glucosidase, alpha-amylase, AChE, BChE was determined. The most active extract was analyzed by validated LC-MS/MS, major compounds were docked with target enzymes. Leaf extract showed the highest phenolics (89.12 microg GAE/mg), flavonoids (1010.67 microg RE/mg), and tannins (152.44 microg TAE/mg). Branch and leaf exhibited strongest antioxidants (DPPH 61.8%/55.5%; ABTS 51.1%/57.2%), surpassing alpha-tocopherol. Branch extract showed the most potent alpha-glucosidase inhibition (IC(50) 107.9 microg/mL vs. acarbose 3081.8 microg/mL). LC-MS/MS revealed high quercetin, epigallocatechin gallate, ellagic, and gallic acids. Results highlight organ-dependent activity and identify plant as a source of natural alpha-glucosidase inhibitors.