Alexander_2010_J.Mol.Neurosci_40_12

Reference

Title : Palmitoylation of nicotinic acetylcholine receptors - Alexander_2010_J.Mol.Neurosci_40_12
Author(s) : Alexander JK , Govind AP , Drisdel RC , Blanton MP , Vallejo Y , Lam TT , Green WN
Ref : Journal of Molecular Neuroscience , 40 :12 , 2010
Abstract :

It is well established that nicotinic acetylcholine receptors (nAChRs) undergo a number of different posttranslational modifications, such as disulfide bond formation, glycosylation, and phosphorylation. Recently, our laboratory has developed more sensitive assays of protein palmitoylation that have allowed us and others to detect the palmitoylation of relatively low abundant proteins such as ligand-gated ion channels. Here, we present evidence that palmitoylation is prevalent on many subunits of different nAChR subtypes, both muscle-type nAChRs and the neuronal "alpha(4)beta(2)" and "alpha(7)" subtypes most abundant in brain. The loss of ligand binding sites that occurs when palmitoylation is blocked with the inhibitor bromopalmitate suggests that palmitoylation of alpha(4)beta(2) and alpha(7) subtypes occurs during subunit assembly and regulates the formation of ligand binding sites. However, additional experiments are needed to test whether nAChR subunit palmitoylation is involved in other aspects of nAChR trafficking or whether palmitoylation regulates nAChR function. Further investigation would be aided by identifying the sites of palmitoylation on the subunits, and here we propose a mass spectrometry strategy for identification of these sites.

PubMedSearch : Alexander_2010_J.Mol.Neurosci_40_12
PubMedID: 19693711

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Citations formats

Alexander JK, Govind AP, Drisdel RC, Blanton MP, Vallejo Y, Lam TT, Green WN (2010)
Palmitoylation of nicotinic acetylcholine receptors
Journal of Molecular Neuroscience 40 :12

Alexander JK, Govind AP, Drisdel RC, Blanton MP, Vallejo Y, Lam TT, Green WN (2010)
Journal of Molecular Neuroscience 40 :12