Alshepy_2025_Chem.Biodivers__e02246

Preparations of plant origin may be used directly for traditional treatment and may also be an origin for drugs used in modern medicine. Within the scope of the study, extracts were obtained from the leaf parts of the Tamarix arabica plant using the maceration method with methanol, ethanol, petroleum ether, and chloroform solvents. Antioxidant capacities of the extracts were determined using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium, 2,2-diphenyl-1-picrylhydrazyl, Cu(2+) reducing capacity, and ferric ion-reducing antioxidant power methods. Moreover, the in vitro effects of extracts on alpha-amylase, alpha-glycosidase, carbonic anhydrase (CA)-I, II, and acetylcholinesterase (AChE) were investigated. At different concentrations, the extract with the highest Fe(3+) and Cu(2+) reduction capacity was ethanol (TAE). While TAE had the most potent activation effect for alpha-amylase, the extracts with the most potent inhibitory effect were TAE, petroleum ether for CA-I, chloroform for CA-II, and, methanol for AChE. The phytochemical content of the methanol extract was determined using liquid chromatography-tandem mass spectrometry. Within the framework of the findings obtained, it may be suggested that the extracts obtained from T. arabica may have antioxidant effects and can be used in the treatment of some diseases due to their effects on some enzyme activities used as therapeutic targets.