Aminoff_2009_Anal.Biochem_392_155

Reference

Title : Research into selective biomarkers of erythrocyte exposure to organophosphorus compounds - Aminoff_2009_Anal.Biochem_392_155
Author(s) : Aminoff D , Bochar DA , Fuller AA , Mapp AK , Showalter HD , Kirchhoff PD
Ref : Analytical Biochemistry , 392 :155 , 2009
Abstract :

Flowcytometric procedures provide distinct advantages over the colorimetric methods currently in use to monitor erythrocytes for exposure of patients to organophosphorus (OP) pesticides and chemical warfare agents; therefore, they warrant exploration. Two types of fluorescent probes-one to detect the total acetylcholinesterase on erythrocytes (RBC-AChE) and the other to distinguish between the active and OP-inhibited RBC-AChE-have been explored. Our studies demonstrate that a fluorescently conjugated fasciculin can be used to monitor total, active, and OP-inhibited RBC-AChE. However, a fluorescently tagged potent inhibitor of AChE, TZ2PIQ-A6 with a K(d) of 33 fM, did not distinguish between the active and OP-inhibited RBC-AChE, nor did three different biotinylated OP compounds. The biotin-fluorescent avidin approach is not a viable procedure for monitoring RBC-AChE. Western blot studies indicate that there are at least 20 serine hydrolases on the surface of red blood cells (RBCs). Plans currently under way for the development of more specific probes to distinguish between active and OP-inhibited RBC-AChE are discussed.

PubMedSearch : Aminoff_2009_Anal.Biochem_392_155
PubMedID: 19497294

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Citations formats

Aminoff D, Bochar DA, Fuller AA, Mapp AK, Showalter HD, Kirchhoff PD (2009)
Research into selective biomarkers of erythrocyte exposure to organophosphorus compounds
Analytical Biochemistry 392 :155

Aminoff D, Bochar DA, Fuller AA, Mapp AK, Showalter HD, Kirchhoff PD (2009)
Analytical Biochemistry 392 :155