| Title : Optimization of enzymatic synthesis of l-arabinose ferulate catalyzed by feruloyl esterases from Myceliophthora thermophila in detergentless microemulsions and assessment of its antioxidant and cytotoxicity activities - Antonopoulou_2018_Process.Biochem_65_100 |
| Author(s) : Antonopoulou I , Papadopoulou A , Iancu L , Cerullo G , Ralli M , Jutten P , Piechot A , Faraco V , Kletsas D , Rova U , ,Christakopoulos P |
| Ref : Process Biochemistry , 48 :100 , 2018 |
|
Abstract :
The feruloyl esterases FaeA1, FaeA2, FaeB1, FaeB2 from Myceliophthora thermophila C1 and MtFae1a from M. thermophila ATCC 42464 were used as biocatalysts for the transesterification of vinyl ferulate (VFA) with l-arabinose in detergentless microemulsions. The effect of parameters such as the microemulsion composition, the substrate concentration, the enzyme load, the pH, the temperature and the agitation was investigated. FaeA1 offered the highest transesterification yield (52.24.3%) after 8h of incubation at 50C using 80mM VFA, 55mM l-arabinose and 0.02mg FAEmL-1 in a mixture comprising of 19.8: 74.7: 5.5v/v/v n-hexane: t-butanol: 100mM MOPS-NaOH pH 8.0. The ability of l-arabinose ferulate (AFA) to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was significant (IC50 386.5M). AFA was not cytotoxic even at high concentrations (1mM) however was found to be pro-oxidant at concentrations higher than 20M when the antioxidant activity was determined with the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay in human skin fibroblasts. |
| PubMedSearch : Antonopoulou_2018_Process.Biochem_65_100 |
| PubMedID: |
| Substrate | L-arabinosyl-ferulate |
Antonopoulou I, Papadopoulou A, Iancu L, Cerullo G, Ralli M, Jutten P, Piechot A, Faraco V, Kletsas D, Rova U, ,Christakopoulos P (2018)
Optimization of enzymatic synthesis of l-arabinose ferulate catalyzed by feruloyl esterases from Myceliophthora thermophila in detergentless microemulsions and assessment of its antioxidant and cytotoxicity activities
Process Biochemistry
48 :100
Antonopoulou I, Papadopoulou A, Iancu L, Cerullo G, Ralli M, Jutten P, Piechot A, Faraco V, Kletsas D, Rova U, ,Christakopoulos P (2018)
Process Biochemistry
48 :100