| Title : Eliminating false-positive results in serum tests for neuromuscular autoimmunity - Apiwattanakul_2010_Muscle.Nerve_41_702 |
| Author(s) : Apiwattanakul M , McKeon A , Pittock SJ , Kryzer TJ , Lennon VA |
| Ref : Muscle & Nerve , 41 :702 , 2010 |
|
Abstract :
In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to 125I-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with 125I-ligand with and without detergent-solubilized cation-channel protein, indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for 125I-ligand binding. |
| PubMedSearch : Apiwattanakul_2010_Muscle.Nerve_41_702 |
| PubMedID: 20405502 |
Apiwattanakul M, McKeon A, Pittock SJ, Kryzer TJ, Lennon VA (2010)
Eliminating false-positive results in serum tests for neuromuscular autoimmunity
Muscle & Nerve
41 :702
Apiwattanakul M, McKeon A, Pittock SJ, Kryzer TJ, Lennon VA (2010)
Muscle & Nerve
41 :702