| Title : Measurement of serum PON-3 concentration: method evaluation, reference values, and influence of genotypes in a population-based study - Aragones_2011_J.Lipid.Res_52_1055 |
| Author(s) : Aragones G , Guardiola M , Barreda M , Marsillach J , Beltran-Debon R , Rull A , Mackness B , Mackness MI , Joven J , Simo JM , Camps J |
| Ref : J Lipid Res , 52 :1055 , 2011 |
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Abstract :
Experimental studies showed that paraoxonase-3 (PON3) retards lipoprotein oxidation. Our objective was to describe a new assay to measure serum PON3 concentrations and report their reference values in a population-based study. The influence of PON3 promoter polymorphisms and their relationships with PON1 and lipid profile were also studied. We generated an anti-PON3 antibody by inoculating rabbits with a synthetic peptide specific to mature PON3. This antibody was used to develop an ELISA. The average regression line of standard plots (n = 8) was y = 0.9587 (0.3392) log(10)x + 1.9466 (0.0861) [r(2) = 0.924 (0.0131); P < 0.001]. There was no cross reaction with PON1. Detection limit was 0.24 mg/l. Imprecision was |
| PubMedSearch : Aragones_2011_J.Lipid.Res_52_1055 |
| PubMedID: 21335322 |
Aragones G, Guardiola M, Barreda M, Marsillach J, Beltran-Debon R, Rull A, Mackness B, Mackness MI, Joven J, Simo JM, Camps J (2011)
Measurement of serum PON-3 concentration: method evaluation, reference values, and influence of genotypes in a population-based study
J Lipid Res
52 :1055
Aragones G, Guardiola M, Barreda M, Marsillach J, Beltran-Debon R, Rull A, Mackness B, Mackness MI, Joven J, Simo JM, Camps J (2011)
J Lipid Res
52 :1055