Arai_2019_J.Biosci.Bioeng_127_222

Reference

Title : Structural characterization of hemicellulose released from corn cob in continuous flow type hydrothermal reactor - Arai_2019_J.Biosci.Bioeng_127_222
Author(s) : Arai T , Biely P , Uhliarikova I , Sato N , Makishima S , Mizuno M , Nozaki K , Kaneko S , Amano Y
Ref : J Biosci Bioeng , 127 :222 , 2019
Abstract :

Hydrothermal reaction is known to be one of the most efficient procedures to extract hemicelluloses from lignocellulosic biomass. We investigated the molecular structure of xylooligosaccharides released from corn cob in a continuous flow type hydrothermal reactor designed in our group. The fraction precipitable from the extract with four volumes of ethanol was examined by (1)H-NMR spectroscopy and MALDI-TOF MS before and after enzymatic treatment with different purified enzymes. The released water-soluble hemicellulose was found to correspond to a mixture of wide degree of polymerization range of acetylarabinoglucuronoxylan fragments (further as corn cob xylan abbreviated CX). Analysis of enzymatic hydrolyzates of CX with an acetylxylan esterase, GH3 beta-xylosidase, GH10 and GH11 xylanases revealed that the main chain contains unsubstituted regions mixed with regions of xylopyranosyl residues partially acetylated and occasionally substituted by 4-O-methyl-d-glucuronic acid and arabinofuranose esterified with ferulic or coumaric acid. Single 2- and 3-O-acetylation was accompanied by 2,3-di-O-acetylation and 3-O-acetylation of Xylp residues substituted with MeGlcA. Most of the non-esterified arabinofuranose side residues were lost during the hydrodynamic process. Despite reduced branching, the acetylation and ferulic acid modification of pentose residues contribute to high yields and high solubility of the extracted CX. It is also shown that different enzyme treatments of CX may lead to various types of xylooligosaccharides of different biomedical potential.

PubMedSearch : Arai_2019_J.Biosci.Bioeng_127_222
PubMedID: 30143337

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Citations formats

Arai T, Biely P, Uhliarikova I, Sato N, Makishima S, Mizuno M, Nozaki K, Kaneko S, Amano Y (2019)
Structural characterization of hemicellulose released from corn cob in continuous flow type hydrothermal reactor
J Biosci Bioeng 127 :222

Arai T, Biely P, Uhliarikova I, Sato N, Makishima S, Mizuno M, Nozaki K, Kaneko S, Amano Y (2019)
J Biosci Bioeng 127 :222