Arnault_1996_J.Mol.Evol_43_109

Reference

Title : Human lipoprotein lipase last exon is not translated, in contrast to lower vertebrates - Arnault_1996_J.Mol.Evol_43_109
Author(s) : Arnault F , Etienne J , Noe L , Raisonnier A , Brault D , Harney JW , Berry MJ , Tse C , Fromental-Ramain C , Hamelin J , Galibert F
Ref : Journal of Molecular Evolution , 43 :109 , 1996
Abstract :

We have sequenced the first fish (zebrafish, Brachydanio rerio) lipoprotein lipase (LPL) cDNA clone. Similarities were found in mammalian LPL cDNA, but the codon spanning the last two exons (which is thus split by the last intron) is AGA (Arg) as opposed to TGA in mammals. Exon 10 is thus partially translated. These results were confirmed with rainbow trout (Oncorhynchus mykiss). We also investigated whether mammal TGA coded for selenocystein (SeCys), the 21st amino acid, but found that this was not the case: TGA does not encode SeCys but is a stop codon. It thus appears that the sense codon AGA (fish) has been transformed into a stop codon TGA (human) during the course of evolution. It remains to be determined if the "loss" of the C-terminal end of mammalian LPL protein has conferred an advantage in terms of LPL activity or, on the contrary, a disadvantage (e.g., susceptibility to diabetes or atherosclerosis).

PubMedSearch : Arnault_1996_J.Mol.Evol_43_109
PubMedID: 8660435
Gene_locus related to this paper: danre-q6p2u2

Related information

Gene_locus danre-q6p2u2

Citations formats

Arnault F, Etienne J, Noe L, Raisonnier A, Brault D, Harney JW, Berry MJ, Tse C, Fromental-Ramain C, Hamelin J, Galibert F (1996)
Human lipoprotein lipase last exon is not translated, in contrast to lower vertebrates
Journal of Molecular Evolution 43 :109

Arnault F, Etienne J, Noe L, Raisonnier A, Brault D, Harney JW, Berry MJ, Tse C, Fromental-Ramain C, Hamelin J, Galibert F (1996)
Journal of Molecular Evolution 43 :109