Ayub_2006_FEMS.Microbiol.Lett_264_125

Reference

Title : Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective beta-ketothiolase gene - Ayub_2006_FEMS.Microbiol.Lett_264_125
Author(s) : Ayub ND , Julia Pettinari M , Mendez BS , Lopez NI
Ref : FEMS Microbiology Letters , 264 :125 , 2006
Abstract :

Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a beta-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous beta-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its beta-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative beta-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids.

PubMedSearch : Ayub_2006_FEMS.Microbiol.Lett_264_125
PubMedID: 17020558

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Citations formats

Ayub ND, Julia Pettinari M, Mendez BS, Lopez NI (2006)
Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective beta-ketothiolase gene
FEMS Microbiology Letters 264 :125

Ayub ND, Julia Pettinari M, Mendez BS, Lopez NI (2006)
FEMS Microbiology Letters 264 :125