| Title : Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A - Bai_2006_Chem.Biol_13_387 |
| Author(s) : Bai L , Li L , Xu H , Minagawa K , Yu Y , Zhang Y , Zhou X , Floss HG , Mahmud T , Deng Z |
| Ref : Chemical Biology , 13 :387 , 2006 |
|
Abstract :
A 45 kb DNA sequencing analysis from Streptomyces hygroscopicus 5008 involved in validamycin A (VAL-A) biosynthesis revealed 16 structural genes, 2 regulatory genes, 5 genes related transport, transposition/integration or tellurium resistance; another 4 genes had no obvious identity. The VAL-A biosynthetic pathway was proposed, with assignment of the required genetic functions confined to the sequenced region. A cluster of eight reassembled genes was found to support VAL-A synthesis in a heterologous host, S. lividans 1326. In vivo inactivation of the putative glycosyltransferase gene (valG) abolished the final attachment of glucose for VAL production and resulted in accumulation of the VAL-A precursor, validoxylamine, while the normal production of VAL-A could be restored by complementation with valG. The role of valG in the glycosylation of validoxylamine to VAL-A was demonstrated in vitro by enzymatic assay. |
| PubMedSearch : Bai_2006_Chem.Biol_13_387 |
| PubMedID: 16632251 |
| Gene_locus related to this paper: strhy-q1l2j3 |
| Gene_locus | strhy-q1l2j3 |
Bai L, Li L, Xu H, Minagawa K, Yu Y, Zhang Y, Zhou X, Floss HG, Mahmud T, Deng Z (2006)
Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A
Chemical Biology
13 :387
Bai L, Li L, Xu H, Minagawa K, Yu Y, Zhang Y, Zhou X, Floss HG, Mahmud T, Deng Z (2006)
Chemical Biology
13 :387