Bai_2015_J.Agric.Food.Chem_63_2198

Reference

Title : Soluble Epoxide Hydrolase Inhibitory and Anti-inflammatory Components from the Leaves of Eucommia ulmoides Oliver (Duzhong) - Bai_2015_J.Agric.Food.Chem_63_2198
Author(s) : Bai MM , Shi W , Tian JM , Lei M , Kim JH , Sun YN , Kim YH , Gao JM
Ref : Journal of Agricultural and Food Chemistry , 63 :2198 , 2015
Abstract :

Eucommia ulmoides leaves have been used as a functional food and drink in China. The purpose of this study was to identify the bioactive constituents with soluble epoxide hydrolase (sEH) inhibitory activity and anti-inflammatory properties. Twenty-seven known compounds (1-27) were isolated from the leaves of E. ulmoides Oliver, and their structures were identified by NMR and ESIMS analysis; three of these, 2,5-dimethoxy-3-glucopyranosyl cinnamic alcohol (11), foliasalacioside E2 (26), and icariside F2 (27), were obtained from this plant for the first time. Compounds 1-7 exhibited soluble epoxide hydrolase (sEH) inhibitory activity at 100 muM; among them, quercetin (1) and kaempferol (5) displayed potential activities with IC50 values of 22.5 +/- 0.9 and 31.3 +/- 2.6 muM, respectively, with noncompetitive inhibition mode. Nuclear factor kappa B (NF-kappaB) inhibitory activity of the isolated compounds was evaluated by the NF-kappaB liciferase assay in HepG2 cells. Compounds 1, 9, 20, and 27 displayed potent NF-kappaB inhibitory effects, with IC50 values of 15.14 +/- 2.29, 15.23 +/- 2.34, 16.88 +/- 2.17, and 16.25 +/- 2.19 muM, respectively, whereas other compounds showed weak inhibition of NF-kappaB transcriptional activity ranging from 17.54 to 92.6 muM. A structure-activity relationship of flavonoids 1-9 was also discussed. The results obtained in this work might contribute to the understanding of pharmacological activities of E. ulmoides leaves and further investigation on its potential application values for food and drug.

PubMedSearch : Bai_2015_J.Agric.Food.Chem_63_2198
PubMedID: 25679330

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Citations formats

Bai MM, Shi W, Tian JM, Lei M, Kim JH, Sun YN, Kim YH, Gao JM (2015)
Soluble Epoxide Hydrolase Inhibitory and Anti-inflammatory Components from the Leaves of Eucommia ulmoides Oliver (Duzhong)
Journal of Agricultural and Food Chemistry 63 :2198

Bai MM, Shi W, Tian JM, Lei M, Kim JH, Sun YN, Kim YH, Gao JM (2015)
Journal of Agricultural and Food Chemistry 63 :2198