Title : Triazophos up-regulated gene expression in the female brown planthopper, Nilaparvata lugens - Bao_2010_J.Insect.Physiol_56_1087 |
Author(s) : Bao YY , Li BL , Liu ZB , Xue J , Zhu ZR , Cheng JA , Zhang CX |
Ref : J Insect Physiol , 56 :1087 , 2010 |
Abstract :
The widespread use of insecticides has caused the resurgence of the brown planthopper, Nilaparvata lugens, in Asia. In this study, we investigated an organo-phosphorous insecticide, triazophos, and its ability to induce gene expression variation in female N. lugens nymphs just before emergence. By using the suppression subtractive hybridization method, a triazophos-induced cDNA library was constructed. In total, 402 differentially expressed cDNA clones were obtained. Real-time qPCR analysis confirmed that triazophos up-regulated the expression of six candidate genes at the transcript level in nymphs on day 3 of the 5th instar. These genes encode N. lugens vitellogenin, bystin, multidrug resistance protein (MRP), purine nucleoside phosphorylase (PNP), pyrroline-5-carboxylate reductase (P5CR) and carboxylesterase. Our results imply that the up-regulation of these genes may be involved in the induction of N. lugens female reproduction or resistance to insecticides. |
PubMedSearch : Bao_2010_J.Insect.Physiol_56_1087 |
PubMedID: 20223245 |
Bao YY, Li BL, Liu ZB, Xue J, Zhu ZR, Cheng JA, Zhang CX (2010)
Triazophos up-regulated gene expression in the female brown planthopper, Nilaparvata lugens
J Insect Physiol
56 :1087
Bao YY, Li BL, Liu ZB, Xue J, Zhu ZR, Cheng JA, Zhang CX (2010)
J Insect Physiol
56 :1087