Barkas_1982_Neurochem.Int_4_361

Reference

Title : Antigenic differences between a proteolipid and a proteodetergent from Torpedo electroplax having similar cholinergic binding properties - Barkas_1982_Neurochem.Int_4_361
Author(s) : Barkas T , De Robertis E , Harrison R , Lunt GG
Ref : Neurochem Int , 4 :361 , 1982
Abstract :

The nicotinic acetylcholine receptor from Torpedo marmorata was extracted and purified from the electroplax membranes by using both aqueous detergent (proteodetergent) or chloroform-methanol (proteolipid). When studied with a highly sensitive radioimmunoassay, it was found that both proteins do not cross-react immunologically against an antireceptor antiserum prepared with the proteodetergent. Treatment with organic solvents of the electroplax membranes, as well as of the proteodetergent receptor purified by affinity chromatography, impaired the radioimmunoassay. This suggests that the antigenicity has been affected by the change in solvent polarity, even though both proteins have similar binding properties for cholinergic ligands. These findings do not allow a simple immunological comparison between the cholinergic proteodetergent and the proteolipid as previously stated in the literature.

PubMedSearch : Barkas_1982_Neurochem.Int_4_361
PubMedID: 20487888

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Citations formats

Barkas T, De Robertis E, Harrison R, Lunt GG (1982)
Antigenic differences between a proteolipid and a proteodetergent from Torpedo electroplax having similar cholinergic binding properties
Neurochem Int 4 :361

Barkas T, De Robertis E, Harrison R, Lunt GG (1982)
Neurochem Int 4 :361