| Title : Classification of the mode of inhibition of high-affinity choline uptake using capillary electrophoresis with electrochemical detection at an enzyme-modified microelectrode - Barkhimer_2005_Anal.Biochem_339_216 |
| Author(s) : Barkhimer TV , Kirchhoff JR , Hudson RA , Messer WS, Jr. |
| Ref : Analytical Biochemistry , 339 :216 , 2005 |
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Abstract :
A nonradiochemical in vitro assay using capillary electrophoresis with electrochemical detection at an enzyme-modified microelectrode has been developed to evaluate the inhibition of high-affinity choline transport in synaptosomes. Quantitative analysis of high-affinity choline transporter rates as a function of inhibitor and substrate concentrations allowed determination of the mode of inhibition for the quaternary ammonium-catechol-based inhibitors 3-[(trimethylammonio)methyl]catechol, N,N-dimethylepinephrine, and 6-hydroxy-N,N-dimethylepinephrine. The results are compared to the well-characterized inhibitor of choline transport, hemicholinium-3. |
| PubMedSearch : Barkhimer_2005_Anal.Biochem_339_216 |
| PubMedID: 15797561 |
Barkhimer TV, Kirchhoff JR, Hudson RA, Messer WS, Jr. (2005)
Classification of the mode of inhibition of high-affinity choline uptake using capillary electrophoresis with electrochemical detection at an enzyme-modified microelectrode
Analytical Biochemistry
339 :216
Barkhimer TV, Kirchhoff JR, Hudson RA, Messer WS, Jr. (2005)
Analytical Biochemistry
339 :216