Title : Nitroxidergic innervation of guinea pig cerebral arteries - Barroso_1996_J.Autonom.Nerv.Syst_58_108 |
Author(s) : Barroso CP , Edvinsson L , Zhang W , Cunha e Sa M , Springall DR , Polak JM , Gulbenkian S |
Ref : Journal of the Autonomic Nervous System , 58 :108 , 1996 |
Abstract :
The presence and distribution of nitric oxide synthase (NOS)-immunoreactive nerve fibers associated with the guinea pig major cerebral arteries was studied by means of immunohistochemical, histochemical and ultrastructural techniques. Anterior arteries of the circle of Willis received a rich supply of perivascular nerve fibers containing NOS immunoreactivity while posteriorly localized arteries presented a moderate to sparse innervation. A double immunofluorescence staining technique revealed that NOS was localized in nerve fibers distinct from those displaying substance P or tyrosine hydroxylase. Combined immunofluorescence and histochemical staining of the same preparation indicated that NOS immunoreactivity was localized in putative cholinergic nerve fibers (identified by their acetylcholinesterase content) and that NADPH-diaphorase activity (a marker for NOS-containing neurons) was found in nerves which also possessed VIP immunoreactivity. The ultrastructural study revealed that NOS immunoreactivity was present in numerous nerve varicosities at the adventitial-medial border. These results suggest that NO and VIP co-exist in putative parasympathetic nerve fibers supplying the guinea pig cerebral arteries and may be release together in response to nervous stimulation. |
PubMedSearch : Barroso_1996_J.Autonom.Nerv.Syst_58_108 |
PubMedID: 8740667 |
Barroso CP, Edvinsson L, Zhang W, Cunha e Sa M, Springall DR, Polak JM, Gulbenkian S (1996)
Nitroxidergic innervation of guinea pig cerebral arteries
Journal of the Autonomic Nervous System
58 :108
Barroso CP, Edvinsson L, Zhang W, Cunha e Sa M, Springall DR, Polak JM, Gulbenkian S (1996)
Journal of the Autonomic Nervous System
58 :108