| Title : M-15: high-affinity chimeric peptide that blocks the neuronal actions of galanin in the hippocampus, locus coeruleus, and spinal cord - Bartfai_1991_Proc.Natl.Acad.Sci.U.S.A_88_10961 |
| Author(s) : Bartfai T , Bedecs K , Land T , Langel U , Bertorelli R , Girotti P , Consolo S , Xu XJ , Wiesenfeld-Hallin Z , Nilsson S , et al. |
| Ref : Proc Natl Acad Sci U S A , 88 :10961 , 1991 |
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Abstract :
The 20-amino acid peptide M-15 binds with high affinity (IC50 approximately 0.1 nM) to 125I-labeled galanin (125I-GAL) binding sites in membranes from the ventral hippocampus, midbrain, and rat spinal cord. Receptor autoradiographic studies show that M-15 can displace 125I-GAL from all labeled sites. M-15 acts as a reversible high-affinity antagonist in blocking the inhibitory effects of GAL on the evoked release of acetylcholine in vivo in the hippocampus and on the GAL-induced hyperpolarization of locus coeruleus neurons in slices. M-15 also blocks the facilitatory effects of GAL on the spinal flexor reflex. Thus, the chimeric peptide M-15 [GAL-(1-13)-substance P-(5-11)amide] represents the first antagonist to the neuronal actions of GAL. |
| PubMedSearch : Bartfai_1991_Proc.Natl.Acad.Sci.U.S.A_88_10961 |
| PubMedID: 1720557 |
Bartfai T, Bedecs K, Land T, Langel U, Bertorelli R, Girotti P, Consolo S, Xu XJ, Wiesenfeld-Hallin Z, Nilsson S, et al. (1991)
M-15: high-affinity chimeric peptide that blocks the neuronal actions of galanin in the hippocampus, locus coeruleus, and spinal cord
Proc Natl Acad Sci U S A
88 :10961
Bartfai T, Bedecs K, Land T, Langel U, Bertorelli R, Girotti P, Consolo S, Xu XJ, Wiesenfeld-Hallin Z, Nilsson S, et al. (1991)
Proc Natl Acad Sci U S A
88 :10961