Baruch_2018_J.Colloid.Interface.Sci_530_328

Reference

Title : Quantitative assessment of paraoxon adsorption to amphiphilic beta-sheet peptides presenting the catalytic triad of esterases - Baruch_2018_J.Colloid.Interface.Sci_530_328
Author(s) : Baruch Leshem A , Isaacs S , Srivastava SK , Abdulhalim I , Kushmaro A , Rapaport H
Ref : J Colloid Interface Sci , 530 :328 , 2018
Abstract :

Organophosphate compounds that are used as pesticides affect the nervous system by binding irreversibly to the active site of the enzyme acetylcholine esterase (AChE) and disrupting neuro-signaling nerve cells. In this study we characterized adsorption of paraoxon to a set of designed peptides that present different arrangements of the three amino acids of the AChE catalytic site: histidine, glutamic-acid and serine. The peptides set included two beta-strands with no net charge and three beta-hairpins that differ in their net charge. Circular dichroism, Thioflavin T assays and TEM images provided only qualitative insights on paraoxon binding to the different peptides. Paraoxon binding to the different peptides was measured with dialysis membrane tubes filled with the peptide solutions and suspended in a reservoir of paraoxon solution. Among all the tested peptides, the single strand peptide, denoted ssESH exhibited at 100muM in random conformation prefibrillar state, the maximum paraoxon adsorption, with a binding mol ratio of one paraoxon per two peptides and an estimated equilibrium binding constant 5 *10(4)M(-1). The three beta-hairpin peptides demonstrated that a net negative charge is unfavorable for paraoxon adsorption. Surface enhanced Raman spectroscopy measurements with ssESH enabled the detection of nanomolar adsorbed concentrations of paraoxon.

PubMedSearch : Baruch_2018_J.Colloid.Interface.Sci_530_328
PubMedID: 29982025

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Citations formats

Baruch Leshem A, Isaacs S, Srivastava SK, Abdulhalim I, Kushmaro A, Rapaport H (2018)
Quantitative assessment of paraoxon adsorption to amphiphilic beta-sheet peptides presenting the catalytic triad of esterases
J Colloid Interface Sci 530 :328

Baruch Leshem A, Isaacs S, Srivastava SK, Abdulhalim I, Kushmaro A, Rapaport H (2018)
J Colloid Interface Sci 530 :328