Bawankar_2013_Phytother.Res_27_864

We prepared a crude gel material from Aloe vera succulent leaf tissues. The ethanolic extract of lyophilized A. vera gel was used for the GC-MS analysis. Hexadecanoic acid (22.22%) was identified as major compound. Sitosterol and stigmasterol were found to be 2.89% and 2.1% in the extract. HPLC analysis was carried out to confirm the presence of stigmasterol. The concentration of sterol extract needed to scavenge DPPH free radical by 50% was calculated as 5.2 mg mL(-1). In the FRAP assay, the sterol extract showed significant hydroxyl radical scavenging in a dose-dependent manner (IC50 value 1.17 microg mL(-1)). Concentration of the sample required to reduce lipid peroxidation was found to be 4.18 microg mL(-1), and the extract also possessed acetylcholinesterase activity (IC50 - 5.26 microg mL(-1)). Catalase activity was 0.196 muM H2 O2 decomposed min(-1) microg(-1) protein, whereas the peroxidase activity was 17.01 muM of pyragallol oxidized min(-1) microg(-1) protein. The extract recorded higher activity against growth of S. greseus and C. albicans in the experiments carried out to determine antibacterial and antifungal activity, respectively.