Belisario-Ferrari_2018_Biotechnol.J__e1800272

Reference

Title : Fast turbidimetric assay for analyzing the enzymatic hydrolysis of polyethylene terephthalate model substrates - Belisario-Ferrari_2018_Biotechnol.J__e1800272
Author(s) : Belisario-Ferrari MR , Wei R , Schneider T , Honak A , Zimmermann W
Ref : Biotechnol J , :e1800272 , 2018
Abstract :

Synthetic plastics such as polyethylene terephthalate (PET) can be cooperatively degraded by microbial polyester hydrolases and carboxylesterases, with the latter hydrolyzing the low-molecular-weight degradation intermediates. For the identification of PET-degrading enzymes, efficient and rapid screening assays are required. Here we report a novel turbidimetric method in a microplate format for the fast screening of enzyme activities against the PET model substrates with two ester bonds bis-(2-Hydroxyethyl) terephthalate (BHET) and ethylene glycol bis-(p-methylbenzoate) (2PET). The carboxylesterase TfCa from Thermobifida fusca KW3 was used for validating the method. High correlation and regression coefficients between the experimental and fitted data confirmed the accuracy and reproducibility of the method and its feasibility for analyzing the kinetics of the enzymatic hydrolysis of the PET model substrates. A comparison of the hydrolysis of BHET and 2PET by TfCa using a kinetic model for heterogeneous catalysis indicated that the enzyme preferentially hydrolyzed the less bulky molecule BHET. The high-throughput assay will facilitate the detection of novel enzymes for the biocatalytic modification or degradation of PET.

PubMedSearch : Belisario-Ferrari_2018_Biotechnol.J__e1800272
PubMedID: 30430764
Gene_locus related to this paper: thefu-1831

Related information

Substrate 2PET    BHETA    BHET
Gene_locus thefu-1831

Citations formats

Belisario-Ferrari MR, Wei R, Schneider T, Honak A, Zimmermann W (2018)
Fast turbidimetric assay for analyzing the enzymatic hydrolysis of polyethylene terephthalate model substrates
Biotechnol J :e1800272

Belisario-Ferrari MR, Wei R, Schneider T, Honak A, Zimmermann W (2018)
Biotechnol J :e1800272