Title : Lead acetate induces hippocampal pyramidal neuron degeneration in mice via up-regulation of executioner caspase-3, oxido-inflammatory stress expression and decreased BDNF and cholinergic activity: Reversal effects of Gingko biloba supplement - Ben-Azu_2021_J.Trace.Elem.Med.Biol_71_126919 |
Author(s) : Ben-Azu B , Adebayo OG , Wopara I , Aduema W , Onyeleonu I , Umoren EB , Kolawole TA , Ebo OT , Akpotu AE , Ajibo DN , Onuoha OG |
Ref : J Trace Elem Med Biol , 71 :126919 , 2021 |
Abstract :
PURPOSE: It has been hypothesized that compounds with strong anti-oxidant activity might mitigate lead-induced neurotoxicity that resulted to neuronal degeneration.Ginkgo biloba supplement (GB-S) is a neuroactive supplement which has been reported to demonstrate neuroprotective effects. In this study, we investigated the reversal effect and the underlying mechanism of GB-S following lead-induced neurotoxicity in mice. METHODS: Male Swiss mice (n = 8) were pre-treated with lead acetate (100 mg/kg) for 30 min before GB-S (10 mg/kg and 20 mg/kg) or Ethylenediaminetetraacetic acid (EDTA) (50 mg/kg) intraperitoneally for 14 consecutive days. Memory impairment symptoms were evaluated on day 13 and 14 using Y-maze and Novel object recognition test (NORT) respectively. Thereafter, spectrophotometry, ELISA, immunohistochemistry and histomorphormetry were used to estimate the degree and expression of biomarkers of neuronal inflammation: oxido-inflammatory stress, apoptosis and degeneration in the hippocampus (HC). RESULTS: Lead acetate treatment significantly (p < 0.05) induced neurobehavioral impairment which was reversed by GB-S as evident in increased percentage alternation and discrimination index. GB-S significantly (p < 0.05) reduced lipid peroxidation and nitrite level, inhibited TNF-alpha and acetylcholinesterase activity and improved glutathione, catalase and superoxide dismutase activity in the HC. Moreover, GB-S inhibited hippocampal apoptosis via elevated expression of caspase-3 with marked increase level of brain derived neurotrophic factor (BDNF). Also, the histomorphormetric study showed that GB-S rescued death of pyramidal neurons (CA3) in the HC. CONCLUSION: Our findings however suggest that GB-S decreased memory impairment progression induced by lead acetate via mechanisms connected to inhibition of oxido-inflammatory stress mediators, restrained acetylcholinesterase activity, up-regulated BDNF/Caspase-3 expression and suppression of hippocampal pyramidal neuron degeneration in mice. |
PubMedSearch : Ben-Azu_2021_J.Trace.Elem.Med.Biol_71_126919 |
PubMedID: 35038618 |
Ben-Azu B, Adebayo OG, Wopara I, Aduema W, Onyeleonu I, Umoren EB, Kolawole TA, Ebo OT, Akpotu AE, Ajibo DN, Onuoha OG (2021)
Lead acetate induces hippocampal pyramidal neuron degeneration in mice via up-regulation of executioner caspase-3, oxido-inflammatory stress expression and decreased BDNF and cholinergic activity: Reversal effects of Gingko biloba supplement
J Trace Elem Med Biol
71 :126919
Ben-Azu B, Adebayo OG, Wopara I, Aduema W, Onyeleonu I, Umoren EB, Kolawole TA, Ebo OT, Akpotu AE, Ajibo DN, Onuoha OG (2021)
J Trace Elem Med Biol
71 :126919