Bensaad_2026_Chem.Biodivers_23_e71578

Natural remedies combined with bioinformatics accelerate the formulation of safer, targeted drugs. This study provides a comprehensive description of the phytochemical and pharmacological aspects of Silene colorata. Binding profiles of eight identified compounds were simulated against alpha-amylase. GC-MS analysis revealed 57 compounds, mainly fatty acids and esters (73.65%), while UHPLC-MS/MS identified 2',4'-dihydroxychalcone (20.72%), (epi)gallocatechin-C-hexoside (12.53%), apigetrin (10.10%), and hexahydroxyheptanoic acid (8.57%) as major components. The hydro-alcoholic extract showed a strong antiradical effect (IC(50) = 7.57 +/- 0.26 microg/mL by ABTS assay; A(0.50) = 48.93 +/- 3.88 microg/mL by phenanthroline assay). Its sun protection factor (SPF) was also the highest (40.561 +/- 0.87). The chloroform fraction showed notable activity against alpha-amylase (IC(50) = 22.86 +/- 3.19 microg/mL) and butyrylcholinesterase (IC(50) = 111.08 +/- 4.72 microg/mL). The complex 3-tetradecyn-1-ol and alpha-amylase appeared more stable, but in an antagonistic manner (-4.8 kcal/mol). While this work reveals crucial findings, further in vivo studies are needed to confirm and expand on these results.