Bi_2012_Planta.Med_78_122

Reference

Title : Fo shou san, an ancient herbal decoction prepared from angelicae sinensis radix and chuanxiong rhizoma, induces erythropoietin expression: a signaling mediated by the reduced degradation of hypoxia-inducible factor in cultured liver cells - Bi_2012_Planta.Med_78_122
Author(s) : Bi CWC , Xu L , Zhang WL , Zhan JY , Fu Q , Zheng KY , Chen VP , Lau DT , Choi RC , Wang TJ , Dong TTX , Tsim KWK
Ref : Planta Med , 78 :122 , 2012
Abstract :

Fo Shou San (FSS) is an ancient herbal decoction composed of Angelicae Sinensis Radix (ASR; Danggui) and Chuanxiong Rhizoma (CR; Chuanxiong) in a ratio of 3:2. FSS is mainly prescribed for patients having a deficiency of blood supply, and it indeed has been shown to stimulate the production of erythropoietin (EPO) in cultured cells. In order to reveal the mechanism of this FSS-induced EPO gene expression, the upstream regulatory cascade, via hypoxia-induced signaling, was revealed here in cultured hepatocellular carcinoma cell line Hep3B. The induction of EPO gene expression, triggered by FSS, was revealed in cultured hepatocytes by: (i) the increase of EPO mRNA; and (ii) the activation of the hypoxia response element (HRE), an upstream regulator of the EPO gene. The FSS-induced EPO gene expression was triggered by an increased expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) protein; however, the mRNA expression of HIF-1 alpha was not altered by the treatment of FSS. The increased HIF-1 alpha was a result of reduced protein degradation after the FSS treatment. The current results therefore provide one of the molecular mechanisms of this ancient herbal decoction for its hematopoietic function.

PubMedSearch : Bi_2012_Planta.Med_78_122
PubMedID: 22095261

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Citations formats

Bi CWC, Xu L, Zhang WL, Zhan JY, Fu Q, Zheng KY, Chen VP, Lau DT, Choi RC, Wang TJ, Dong TTX, Tsim KWK (2012)
Fo shou san, an ancient herbal decoction prepared from angelicae sinensis radix and chuanxiong rhizoma, induces erythropoietin expression: a signaling mediated by the reduced degradation of hypoxia-inducible factor in cultured liver cells
Planta Med 78 :122

Bi CWC, Xu L, Zhang WL, Zhan JY, Fu Q, Zheng KY, Chen VP, Lau DT, Choi RC, Wang TJ, Dong TTX, Tsim KWK (2012)
Planta Med 78 :122