Bianchini_2022_Chem.Biol.Interact__110121

Reference

Title : MeHg exposure impairs both the catecholaminergic and cholinergic systems resulting in motor and non-motor behavioral changes in Drosophila melanogaster - Bianchini_2022_Chem.Biol.Interact__110121
Author(s) : Bianchini MC , Soares LFW, Jr. , Sousa J , Ramborger BP , Gayer MC , Bridi JC , Roehrs R , Pinton S , Aschner M , Avila DS , Puntel RL
Ref : Chemico-Biological Interactions , :110121 , 2022
Abstract :

Human exposure to the natural environmental contaminant methylmercury (MeHg) has been associated to adverse health effects. Importantly, the mechanisms by which this organomercurial exerts its neurotoxicity have yet to be fully clarified. Therefore, the aim of this study was to evaluate whether exposure to MeHg alters dopamine (DA) and octopamine (OA) levels, acetylcholinesterase (AChE) activity and impacts both motor and non-motor behaviours. We studied the effect of MeHg by feeding 1-2 d old flies (male and females) with 25 and 50 microM MeHg for 4 d and determined effects on survival, motor and non-motor behaviours, oxidative stress, AChE and tyrosine hydroxylase (TH) activities, as well as DA and OA levels. We found that Drosophila melanogaster (D. melanogaster) exposed to MeHg showed a reduction in survival rate, associated with the inhibition of AChE and TH activities in head of flies and decreased DA and OA levels. These changes were accompanied by behavioural alterations, such as locomotor deficit and increased grooming behaviour, in addition to an increase in oxidative stress markers both in head and in body of flies, and an increase in glutathione-S-transferase (GST) activity in head of flies. Collectively, our data support the hypothesis that MeHg neurotoxicity is associated with altered OA and DA levels, AChE inhibition, which may serve, at least in part, as the underpinnings of both motor and non-motor behavioural changes.

PubMedSearch : Bianchini_2022_Chem.Biol.Interact__110121
PubMedID: 35995257

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Citations formats

Bianchini MC, Soares LFW, Jr., Sousa J, Ramborger BP, Gayer MC, Bridi JC, Roehrs R, Pinton S, Aschner M, Avila DS, Puntel RL (2022)
MeHg exposure impairs both the catecholaminergic and cholinergic systems resulting in motor and non-motor behavioral changes in Drosophila melanogaster
Chemico-Biological Interactions :110121

Bianchini MC, Soares LFW, Jr., Sousa J, Ramborger BP, Gayer MC, Bridi JC, Roehrs R, Pinton S, Aschner M, Avila DS, Puntel RL (2022)
Chemico-Biological Interactions :110121