| Title : Glucuronoyl esterases are active on the polymeric substrate methyl esterified glucuronoxylan - Biely_2015_FEBS.Lett_589_2334 |
| Author(s) : Biely P , Malovikova A , Uhliarikova I , Li XL , Wong DWS |
| Ref : FEBS Letters , 589 :2334 , 2015 |
| Abstract : Alkali extracted beechwood glucuronoxylan methyl ester prepared by esterification of 4-O-methyl-d-glucuronic acid side residues by methanol was found to serve as substrate of microbial glucuronoyl esterases from Ruminococcus flavefaciens, Schizophyllum commune and Trichoderma reesei. The enzymatic deesterification was monitored by (1)H NMR spectroscopy and evaluated on the basis of the decrease of the signal of the ester methyl group and increase of the signal of methanol. The results show for the first time the action of enzymes on polymeric substrate, which imitates more closely the natural substrate in plant cell walls than the low molecular mass artificial substrates used up to present. |
| ESTHER : Biely_2015_FEBS.Lett_589_2334 |
| PubMedSearch : Biely_2015_FEBS.Lett_589_2334 |
| PubMedID: 26216754 |
| Gene_locus related to this paper: rumfl-CESA |
| Gene_locus related to this paper: rumfl-CESA |
Biely P, Malovikova A, Uhliarikova I, Li XL, Wong DWS (2015)
Glucuronoyl esterases are active on the polymeric substrate methyl esterified glucuronoxylan
FEBS Letters
589 :2334
Biely P, Malovikova A, Uhliarikova I, Li XL, Wong DWS (2015)
FEBS Letters
589 :2334