Billig_2010_Appl.Microbiol.Biotechnol_87_1753

Reference

Title : Hydrolysis of cyclic poly(ethylene terephthalate) trimers by a carboxylesterase from Thermobifida fusca KW3 - Billig_2010_Appl.Microbiol.Biotechnol_87_1753
Author(s) : Billig S , Oeser T , Birkemeyer C , Zimmermann W
Ref : Applied Microbiology & Biotechnology , 87 :1753 , 2010
Abstract :

We have identified a carboxylesterase produced in liquid cultures of the thermophilic actinomycete Thermobifida fusca KW3 that were supplemented with poly(ethylene terephthalate) fibers. The enzyme hydrolyzed highly hydrophobic, synthetic cyclic poly(ethylene terephthalate) trimers with an optimal activity at 60 degrees C and a pH of 6. V (max) and K (m) values for the hydrolysis were 9.3 micromol(-1) min(-1) mg(-1) and 0.5 mM, respectively. The esterase showed high specificity towards short and middle chain-length fatty acyl esters of p-nitrophenol. The enzyme retained 37% of its activity after 96 h of incubation at 50 degrees C and a pH of 8. Enzyme inhibition studies and analysis of substitution mutants of the carboxylesterase revealed the typical catalytic mechanism of a serine hydrolase with a catalytic triad composed of serine, glutamic acid, and histidine.

PubMedSearch : Billig_2010_Appl.Microbiol.Biotechnol_87_1753
PubMedID: 20467738
Gene_locus related to this paper: thefu-1831

Citations formats

Billig S, Oeser T, Birkemeyer C, Zimmermann W (2010)
Hydrolysis of cyclic poly(ethylene terephthalate) trimers by a carboxylesterase from Thermobifida fusca KW3
Applied Microbiology & Biotechnology 87 :1753

Billig S, Oeser T, Birkemeyer C, Zimmermann W (2010)
Applied Microbiology & Biotechnology 87 :1753