| Title : Occurrence of fatty acid epoxide hydrolases in soybean (Glycine max). Purification and characterization of the soluble form - Blee_1992_Biochem.J_282 ( Pt 3)_711 |
| Author(s) : Blee E , Schuber F |
| Ref : Biochemical Journal , 282 ( Pt 3) :711 , 1992 |
|
Abstract :
Epoxide hydrolases catalysing the hydration of cis-9,10-epoxystearate into threo-9,10-dihydroxystearate have been detected in soybean (Glycine max) seedlings. The major activity was found in the cytosol, a minor fraction being strongly associated with microsomes. The soluble enzyme, which was purified to apparent homogeneity by (NH4)2SO4 fractionation, hydrophobic, DEAE- and gel-filtration chromatographies, has a molecular mass of 64 kDa and a pI of 5.4. |
| PubMedSearch : Blee_1992_Biochem.J_282 ( Pt 3)_711 |
| PubMedID: 1554352 |
Blee E, Schuber F (1992)
Occurrence of fatty acid epoxide hydrolases in soybean (Glycine max). Purification and characterization of the soluble form
Biochemical Journal
282 ( Pt 3) :711
Blee E, Schuber F (1992)
Biochemical Journal
282 ( Pt 3) :711