Blodgett_2005_Antimicrob.Agents.Chemother_49_230

Reference

Title : Molecular cloning, sequence analysis, and heterologous expression of the phosphinothricin tripeptide biosynthetic gene cluster from Streptomyces viridochromogenes DSM 40736 - Blodgett_2005_Antimicrob.Agents.Chemother_49_230
Author(s) : Blodgett JA , Zhang JK , Metcalf WW
Ref : Antimicrobial Agents & Chemotherapy , 49 :230 , 2005
Abstract :

A fosmid library from genomic DNA of Streptomyces viridochromogenes DSM 40736 was constructed and screened for the presence of genes known to be involved in the biosynthesis of phosphinothricin tripeptide (PTT). Eight positives were identified, one of which was able to confer PTT biosynthetic capability upon Streptomyces lividans after integration of the fosmid into the chromosome of this heterologous host. Sequence analysis of the 40,241-bp fosmid insert revealed 29 complete open reading frames (ORFs). Deletion analysis demonstrated that a minimum set of 24 ORFs were required for PTT production in the heterologous host. Sequence analysis revealed that most of these PTT genes have been previously identified in either S. viridochromogenes or S. hygroscopicus (or both), although only 11 out of 24 of these ORFs have experimentally defined functions. Three previously unknown genes within the cluster were identified and are likely to have roles in the stepwise production of phosphonoformate from phosphonoacetaldehyde. This is the first report detailing the entire PTT gene cluster from any producing streptomycete.

PubMedSearch : Blodgett_2005_Antimicrob.Agents.Chemother_49_230
PubMedID: 15616300
Gene_locus related to this paper: strvi-naptd , strvr-q5iw44 , strvr-q5iw45

Related information

Gene_locus strvi-naptd    strvr-q5iw44    strvr-q5iw45

Citations formats

Blodgett JA, Zhang JK, Metcalf WW (2005)
Molecular cloning, sequence analysis, and heterologous expression of the phosphinothricin tripeptide biosynthetic gene cluster from Streptomyces viridochromogenes DSM 40736
Antimicrobial Agents & Chemotherapy 49 :230

Blodgett JA, Zhang JK, Metcalf WW (2005)
Antimicrobial Agents & Chemotherapy 49 :230