Bock_1980_J.Neurochem_35_1297

Reference

Title : Nervous system-specific proteins in developing rat cerebral cells in culture - Bock_1980_J.Neurochem_35_1297
Author(s) : Bock E , Yavin Z , Jorgensen OS , Yavin E
Ref : Journal of Neurochemistry , 35 :1297 , 1980
Abstract :

The nervous system-specific proteins; synaptin, D1, D2, D3, glial fibrillary acidic protein (GFA) and 14-3-2, were quantified in dissociated cerebral cells from the foetal rat brain at various times of growth in culture. By approximately 1 week in culture, the neuronal membrane markers synaptin, D1, D2, and D3 could all be demonstrated. A maximum concentration of 10-20% for synaptin, D1, and D3 and 160% for D2, in comparison with the levels in adult forebrain, was attained during the 2nd week in vitro. The astroglial gliofilament marker GFA increased continuously, reaching by 38 days of cultivation an 18-fold higher level than the concentration in adult forebrain. The neuronal cytoplasm marker 14-3-2 could be demonstrated in trace amounts, and only after more than 1 week in vitro. Neuronal cell bodies and processes stained by indirect immunofluorescence using an anti-D2 serum were strongly fluorescent after 1 week in vitro. Immunofluorescence staining for GFA revealed a cytoplasmic filamentous network in perinuclear areas and processes of, presumably, astroblasts.

PubMedSearch : Bock_1980_J.Neurochem_35_1297
PubMedID: 7003063

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Citations formats

Bock E, Yavin Z, Jorgensen OS, Yavin E (1980)
Nervous system-specific proteins in developing rat cerebral cells in culture
Journal of Neurochemistry 35 :1297

Bock E, Yavin Z, Jorgensen OS, Yavin E (1980)
Journal of Neurochemistry 35 :1297