Bondzic_2020_Eur.J.Pharm.Sci_151_105376

Reference

Title : A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules - the role in the mechanism of AChE inhibition - Bondzic_2020_Eur.J.Pharm.Sci_151_105376
Author(s) : Bondzic AM , Lazarevic-Pasti TD , Leskovac AR , Petrovic SZ , Colovic MB , Parac-Vogt TN , Janjic GV
Ref : Eur J Pharm Sci , 151 :105376 , 2020
Abstract :

Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors, 12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate (POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism and tryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significant changes in the secondary structure of the enzyme. The molecular docking approach has revealed a new allosteric binding site, termed beta-allosteric site (beta-AS), which is considered responsible for the inhibition of AChE by POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is considered responsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selected POMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. It was shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, which indicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable of binding to an allosteric site that so far has not been considered responsible for enzyme inhibition could be fundamental for the development of new drug design strategies and the discovery of more efficient AChE modulators.

PubMedSearch : Bondzic_2020_Eur.J.Pharm.Sci_151_105376
PubMedID: 32492460

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Citations formats

Bondzic AM, Lazarevic-Pasti TD, Leskovac AR, Petrovic SZ, Colovic MB, Parac-Vogt TN, Janjic GV (2020)
A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules - the role in the mechanism of AChE inhibition
Eur J Pharm Sci 151 :105376

Bondzic AM, Lazarevic-Pasti TD, Leskovac AR, Petrovic SZ, Colovic MB, Parac-Vogt TN, Janjic GV (2020)
Eur J Pharm Sci 151 :105376