Born_2014_Proc.Natl.Acad.Sci.U.S.A_111_E1274

Reference

Title : Modulation of synaptic function through the alpha-neurexin-specific ligand neurexophilin-1 - Born_2014_Proc.Natl.Acad.Sci.U.S.A_111_E1274
Author(s) : Born G , Breuer D , Wang S , Rohlmann A , Coulon P , Vakili P , Reissner C , Kiefer F , Heine M , Pape HC , Missler M
Ref : Proc Natl Acad Sci U S A , 111 :E1274 , 2014
Abstract : Neurotransmission at different synapses is highly variable, and cell-adhesion molecules like alpha-neurexins (alpha-Nrxn) and their extracellular binding partners determine synapse function. Although alpha-Nrxn affect transmission at excitatory and inhibitory synapses, the contribution of neurexophilin-1 (Nxph1), an alpha-Nrxn ligand with restricted expression in subpopulations of inhibitory neurons, is unclear. To reveal its role, we investigated mice that either lack or overexpress Nxph1. We found that genetic deletion of Nxph1 impaired GABAB receptor (GABABR)-dependent short-term depression of inhibitory synapses in the nucleus reticularis thalami, a region where Nxph1 is normally expressed at high levels. To test the conclusion that Nxph1 supports presynaptic GABABR, we expressed Nxph1 ectopically at excitatory terminals in the neocortex, which normally do not contain this molecule but can be modulated by GABABR. We generated Nxph1-GFP transgenic mice under control of the Thy1.2 promoter and observed a reduced short-term facilitation at these excitatory synapses, representing an inverse phenotype to the knockout. Consistently, the diminished facilitation could be reversed by pharmacologically blocking GABABR with CGP-55845. Moreover, a complete rescue was achieved by additional blocking of postsynaptic GABAAR with intracellular picrotoxin or gabazine, suggesting that Nxph1 is able to recruit or stabilize both presynaptic GABABR and postsynaptic GABAAR. In support, immunoelectron microscopy validated the localization of ectopic Nxph1 at the synaptic cleft of excitatory synapses in transgenic mice and revealed an enrichment of GABAAR and GABABR subunits compared with wild-type animals. Thus, our data propose that Nxph1 plays an instructive role in synaptic short-term plasticity and the configuration with GABA receptors.
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PubMedSearch : Born_2014_Proc.Natl.Acad.Sci.U.S.A_111_E1274
PubMedID: 24639499

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Citations formats

Born G, Breuer D, Wang S, Rohlmann A, Coulon P, Vakili P, Reissner C, Kiefer F, Heine M, Pape HC, Missler M (2014)
Modulation of synaptic function through the alpha-neurexin-specific ligand neurexophilin-1
Proc Natl Acad Sci U S A 111 :E1274

Born G, Breuer D, Wang S, Rohlmann A, Coulon P, Vakili P, Reissner C, Kiefer F, Heine M, Pape HC, Missler M (2014)
Proc Natl Acad Sci U S A 111 :E1274