Boudjema_2024_Chem.Biodivers__e202402709

The current study was conducted to explore the phytochemical composition and in vitro antioxidant activities of Moringa oleifera (MO) leaf aqueous extract, as well as its in vivo modulatory effects on abamectin (ABM) induced oxidative stress in rat erythrocytes and brain. Animals were randomly divided into four groups. The first group served as a control and received distilled water by gavage. The second group (ABM) received abamectin (1 mg/kg b.w. in drinking water). The third group (MO) received M. oleifera leaf aqueous extract (200 mg/kg b.w. by gavage). The fourth group (ABM-MO) received a combination of ABM and MO. ABM inhibited acetylcholinesterase activity, decreased the activities of antioxidant enzymes. Supplementation with MO in ABM-treated rats significantly ameliorated the biochemical parameters cited above. The computational modeling revealed that M. oleifera identified compounds bound human peroxiredoxin 5, catalase and glutathione peroxidase with acceptable affinities, which together with the established molecular interactions and tight embedding satisfactory support the in vivo results. Thus, it may be concluded that ABM impairs brain and erythrocyte function through oxidative damage, and these effects could be prevented by Moringa oleifera leaf aqueous extract, likely due to its antioxidant activity.