Boulin_2012_Nat.Neurosci_15_1374

Reference

Title : Positive modulation of a Cys-loop acetylcholine receptor by an auxiliary transmembrane subunit - Boulin_2012_Nat.Neurosci_15_1374
Author(s) : Boulin T , Rapti G , Briseno-Roa L , Stigloher C , Richmond JE , Paoletti P , Bessereau JL
Ref : Nat Neurosci , 15 :1374 , 2012
Abstract :

Auxiliary subunits regulate the trafficking, localization or gating kinetics of voltage- and ligand-gated ion channels by associating tightly and specifically with pore-forming subunits. However, no auxiliary subunits have been identified for members of the Cys-loop receptor superfamily. Here we identify MOLO-1, a positive regulator of levamisole-sensitive acetylcholine receptors (L-AChRs) at the Caenorhabditis elegans neuromuscular junction. MOLO-1 is a one-pass transmembrane protein that contains a single extracellular globular domain-the TPM domain, found in bacteria, plants and invertebrates, including nonvertebrate chordates. Loss of MOLO-1 impairs locomotion and renders worms resistant to the anthelmintic drug levamisole. In molo-1 mutants, L-AChR-dependent synaptic transmission is reduced by half, while the number and localization of receptors at synapses remain unchanged. In a heterologous expression system, MOLO-1 physically interacts with L-AChRs and directly enhances channel gating without affecting unitary conductance. The identification of MOLO-1 expands the mechanisms for generating functional and pharmacological diversity in the Cys-loop superfamily.

PubMedSearch : Boulin_2012_Nat.Neurosci_15_1374
PubMedID: 22922783

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Citations formats

Boulin T, Rapti G, Briseno-Roa L, Stigloher C, Richmond JE, Paoletti P, Bessereau JL (2012)
Positive modulation of a Cys-loop acetylcholine receptor by an auxiliary transmembrane subunit
Nat Neurosci 15 :1374

Boulin T, Rapti G, Briseno-Roa L, Stigloher C, Richmond JE, Paoletti P, Bessereau JL (2012)
Nat Neurosci 15 :1374